| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,004,376 | T→C | intergenic (+16/‑60) | yigL → / → bioP | sugar/pyridoxal phosphate phosphatase YigL/biotin transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,004,376 | 0 | T | C | 98.0% | 178.9 / ‑5.0 | 51 | intergenic (+16/‑60) | yigL/bioP | sugar/pyridoxal phosphate phosphatase YigL/biotin transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (29/20); total (31/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATT > NZ_CP009273/4004289‑4004462 | gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCa > 1:1066449/1‑90 (MQ=255)gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCa < 2:488873/90‑1 (MQ=255)gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCa < 1:977838/90‑1 (MQ=255)gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCa > 1:880103/1‑90 (MQ=255) gAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTc < 2:1066449/90‑1 (MQ=255) gAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTc < 1:576571/90‑1 (MQ=255) gATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCt > 1:124483/1‑90 (MQ=255) ggTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATa > 1:618236/1‑90 (MQ=255) tAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCg > 1:104454/1‑90 (MQ=255) aaTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTc < 2:217320/75‑1 (MQ=255) aaTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTc > 1:523326/1‑75 (MQ=255) aaTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTc > 1:217320/1‑75 (MQ=255) aaTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTc < 2:523326/75‑1 (MQ=255) gCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTAc < 1:227567/90‑1 (MQ=255) cgacgaCGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACaa > 1:663971/1‑90 (MQ=255) cgacgaCGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACaa > 1:741747/1‑90 (MQ=255) gCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCACCCTGATACTTCGCTACAATGGATACCCGtt < 2:502245/90‑1 (MQ=255) gCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAAt > 2:999844/1‑90 (MQ=255) ttATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCaa > 2:755301/1‑90 (MQ=255) aTCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATCCTTGGCTAAAATGGATACCCGTTAATCAAag > 2:801987/1‑90 (MQ=255) aTCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATa < 1:674975/76‑1 (MQ=255) aTCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATa > 2:674975/1‑76 (MQ=255) aTCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAag > 1:824552/1‑90 (MQ=255) aTCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAag < 1:85229/90‑1 (MQ=255) cTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAagag > 2:962410/1‑90 (MQ=255) tGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGt > 2:526127/1‑90 (MQ=255) tGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGt < 2:950012/90‑1 (MQ=255) gCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGtt > 2:149436/1‑90 (MQ=255) cGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGttt > 1:39028/1‑90 (MQ=255) gTAAACTCTATTTATCGTAATCGTTCTTTATTTGGACAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGtttt > 2:107472/1‑90 (MQ=255) ctctATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCAtt < 1:235039/90‑1 (MQ=255) tctATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATtg < 1:156736/90‑1 (MQ=255) tATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGtt < 2:922367/68‑1 (MQ=255) tATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGtt > 1:922367/1‑68 (MQ=255) tATTTATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATtgtg > 1:486134/1‑90 (MQ=255) ttATCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcg < 1:749169/90‑1 (MQ=255) aTCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGTCGCt < 1:624871/90‑1 (MQ=255) tCGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTa > 2:675863/1‑90 (MQ=255) cGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTa < 1:117074/62‑1 (MQ=255) cGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTa > 2:117074/1‑62 (MQ=255) cGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTAc > 1:26883/1‑90 (MQ=255) cGTAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTAc < 2:741747/90‑1 (MQ=255) tAATCGTTCTTTATTTGGCCGGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACtt > 1:399720/1‑90 (MQ=255) tAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACtt > 2:386854/1‑90 (MQ=255) tAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACtt > 2:730006/1‑90 (MQ=255) tAATCGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACtt > 2:784399/1‑90 (MQ=255) cGTTCTTTATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTatca < 2:177521/90‑1 (MQ=255) ttATTTGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATcacca > 2:63573/1‑90 (MQ=255) ttGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAt < 2:486134/90‑1 (MQ=255) ttGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAt > 2:219455/1‑90 (MQ=255) tGGCCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAATGATTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt > 2:153291/1‑90 (MQ=255) | GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATT > NZ_CP009273/4004289‑4004462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |