| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 392,218 | T→C | S42P (TCC→CCC) | sbmA → | peptide antibiotic transporter SbmA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 392,218 | 0 | T | C | 96.0% | 77.1 / ‑3.8 | 25 | S42P (TCC→CCC) | sbmA | peptide antibiotic transporter SbmA |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (12/12); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
TTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTT > NZ_CP009273/392131‑392304 | tttCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTccc < 1:565624/90‑1 (MQ=255) tcatttGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCa > 2:472410/4‑82 (MQ=255) tcatttGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCa < 1:472410/79‑1 (MQ=255) gggCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAgcg > 1:126176/1‑90 (MQ=255) attgattgCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGcgccgc > 1:834274/1‑90 (MQ=255) tgatttCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCcgcgc < 2:836050/90‑1 (MQ=255) ccGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCt < 1:169380/90‑1 (MQ=255) ccGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCt > 1:941237/1‑90 (MQ=255) ggCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCCCCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGAtt < 2:343873/90‑1 (MQ=255) aaGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTcc > 1:299523/1‑90 (MQ=255) aaGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTcc > 2:695475/1‑90 (MQ=255) aGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCt > 1:807351/1‑90 (MQ=255) cGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAt > 2:685020/1‑90 (MQ=255) gggTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTa < 2:299523/90‑1 (MQ=255) gggTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTa < 1:459963/90‑1 (MQ=255) gTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACg < 2:834274/90‑1 (MQ=255) gTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACg < 1:695475/90‑1 (MQ=255) gTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACg < 1:506352/90‑1 (MQ=255) gTGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGATCGTTGGATTTCCTGATTTTTTACg < 2:340542/90‑1 (MQ=255) tGACTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGc > 2:552353/1‑90 (MQ=255) cTGGGTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTta > 1:1045964/1‑90 (MQ=255) ggTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtacta > 2:895846/1‑90 (MQ=255) gTGGCGCGTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTtactac > 2:1010187/1‑90 (MQ=255) gcgTATCACCGGCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGt > 1:93174/1‑90 (MQ=255) ggCGCTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGa < 1:572677/90‑1 (MQ=255) gcTCCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACtt < 1:729956/90‑1 (MQ=255) | TTTCTCTCGGCCTTCGTTTGGGCATTGATTGCCGTTATCTTCTGGCAAGCCGGTGGGGGTGACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTT > NZ_CP009273/392131‑392304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |