| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,571,380 | 0 | A | C | 61.1% | 0.4 / 13.5 | 18 | V166G (GTA→GGA) | yddB | TonB‑dependent receptor plug domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/11); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAATA > NZ_CP009273/1571295‑1571465 | ttttAACCAGTCCGAACGCGTAGTGCGATACCCCAATTTCCCCTTGCTATCATCAGCGTTGAAGCTTTTGATCTTTGCATCAATT‑Ccccc < 1:268586/88‑1 (MQ=255) gTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACTTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCg > 2:211803/1‑90 (MQ=255) gTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCGCCATTGaa > 2:181041/1‑90 (MQ=255) cgCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCGCCATTGAAGCGACCAAATTCaa > 2:251055/2‑90 (MQ=255) aTACCCCAATTTCCCCTTGCTATCATCAGCGTTGAAGCGTTTAATCTTTGCATAAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCgg < 1:251055/90‑1 (MQ=255) aTTTCCCCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAAc < 2:40405/90‑1 (MQ=255) tttCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACt < 2:119038/90‑1 (MQ=255) ttCCCCTTGCTATCATCGGCGTTGAAGCTTTTGTTTTTTGCATCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTg < 1:247760/90‑1 (MQ=255) ttGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa > 1:330260/1‑90 (MQ=255) ttGCTATCACCAGCGTTGAAGCTTTTGATTTTTGAATAAATC‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa < 1:211803/90‑1 (MQ=255) atcaGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTcac > 2:124035/1‑90 (MQ=255) caGCGTTGAGGCTTTTGATTTTTCCACCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 1:136665/90‑1 (MQ=255) aGCGTTGAAGTTTTTGATCTTTCCATAAATTCCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 1:132056/90‑1 (MQ=255) aGCGTTGAAGCTTTTGATTTTTGCATCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAtt < 2:330260/90‑1 (MQ=255) ttttttGCACCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACa < 2:206403/87‑1 (MQ=255) ttGCATCAATT‑CCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCaa < 2:272211/90‑1 (MQ=255) tgccATCAATT‑ACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCaa < 2:23461/87‑1 (MQ=255) attt‑ACCCCGCCATTGGAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGCAAGCTGACATCAAGataata < 1:199296/88‑1 (MQ=255) | TGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGATCTTTGCATCAATT‑ACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAATA > NZ_CP009273/1571295‑1571465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |