Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,601,6030TG52.6% 5.8 / 13.4 19V443G (GTG→GGG) hyfFhydrogenase 4 subunit F
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base G (9/1);  total (13/6)
Fisher's exact test for biased strand distribution p-value = 5.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCAT  >  NZ_CP009273/2601531‑2601687
                                                                        |                                                                                    
tgctgATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGGGTTAATGGCGAAAccgc                                                                     >  1:65236/1‑90 (MQ=255)
 gctgATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGGGTTAATGGCGGAAccgcc                                                                    >  1:58696/1‑90 (MQ=255)
              ctgctgTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTTGTACGGATGGCTGCGGGGGGGTTAATGGCGAAACCCCCCCAGGCCGTTAAc                                                       >  2:293487/1‑90 (MQ=255)
               tgctgTTATGGCTGTTAACGTGGGTGCGGGGGGGCCTGGTCCGGATGGCTGCGGGGGTTTTAATGGCAAAACCGCCGCAGGCCGTTAAcc                                                      <  1:213144/90‑1 (MQ=255)
                gctgTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGGACGGATGGCTGCGCGGGGGTTAACGGCGAAACCGCCGCAGGCCGGTAACCg                                                     >  1:280260/1‑90 (MQ=255)
                     tATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGGGGGGGGGTAATGGGGGAAACCCCCCAGGGCGGTAAACggggggg                                               >  1:157520/1‑87 (MQ=255)
                     tATTGCTGTTAACGCTGGTGCTGGCGGGCCCGGGACGGGTGGCTGCGCGGGTGTTAAAGGGGGAACCGCCCCCGGGCGGTAAACgggggg                                                >  1:44375/1‑88 (MQ=255)
                             ttAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGGGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGGGATCTCGGc                                        >  2:61568/1‑90 (MQ=255)
                                   cTGGTGCTGGCGGGCCTGGGACGGATGGCTGCGCGGGGGGTAATGGGGGAACCCCCCCAGGCCGGTAAACGGGGGGGTTTTGGCTGGGTg                                  >  1:177443/1‑90 (MQ=255)
                                    tGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGGGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCCGCTGGGTGa                                 >  2:228857/1‑90 (MQ=255)
                                    tGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGGGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGGGATCTCGGCTGGTTGa                                 >  2:35660/1‑90 (MQ=255)
                                               ggCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCa                      <  2:177443/90‑1 (MQ=255)
                                                  cTGGTCCGGTTGGCTGCGCGGGGTTTATGGGGGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGCCCACCTCGCCAATg                   <  1:326396/90‑1 (MQ=255)
                                                          gATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTct           >  1:161011/1‑90 (MQ=255)
                                                            tGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTctgc         <  2:65236/90‑1 (MQ=255)
                                                             ggCTGCGCGGGTGTTAATGGCGAACCCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGaccacc                            <  1:156425/70‑1 (MQ=255)
                                                             ggCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGaccacc                            >  2:156425/1‑70 (MQ=255)
                                                              gCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTctgctg       >  2:78954/1‑90 (MQ=255)
                                                                   gcgGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCat  <  2:157520/90‑1 (MQ=255)
                                                                        |                                                                                    
TGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCAT  >  NZ_CP009273/2601531‑2601687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: