| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,775,223 | 0 | T | G | 52.9% | 11.4 / 14.4 | 17 | T234P (ACC→CCC) | cysE | serine O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTCGCGCCC > NZ_CP009273/3775140‑3775311 | gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGTTgcgg > 1:283363/1‑90 (MQ=255)gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATgcgg > 1:126926/1‑90 (MQ=255) ttGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATggggg > 1:318274/1‑89 (MQ=255) ttGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATgcggc > 1:107963/1‑90 (MQ=255) gCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGTTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCCCCg > 2:244959/1‑90 (MQ=255) tGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGGTGGGGCGGGAcccgg > 1:79017/1‑87 (MQ=255) tatCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTgcagca < 2:301776/90‑1 (MQ=255) aTTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGTGGGTGCGGCGGCACCGCGTTCAGCACCCCg > 1:264826/1‑90 (MQ=255) tGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATGCGGCGGCACCGGGTGCAGCACCACGGaaac > 2:91109/1‑88 (MQ=255) tCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCa < 1:135431/90‑1 (MQ=255) cTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATc > 2:87516/1‑90 (MQ=255) tACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACg < 1:111882/65‑1 (MQ=255) tACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACg > 2:111882/1‑65 (MQ=255) aCAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCa < 1:168660/90‑1 (MQ=255) cGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCCGGTGCAGCACCCCGGAGCCCGCGCCCATCTTCCCCCCGCGCCCCACTTCa > 1:104422/1‑90 (MQ=255) gCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCGGTTGCAGCACCACGGAACCCGCGCCAATCTTCGCGCCGCGCCCCACTTCAata > 1:49169/1‑90 (MQ=255) ccGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAatat < 1:152925/90‑1 (MQ=255) gtATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTcgcgccc < 2:280550/90‑1 (MQ=255) | GTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTCGCGCCC > NZ_CP009273/3775140‑3775311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |