| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 341,015 | 0 | G | A | 36.0% | 23.5 / 10.2 | 25 | intergenic (+313/‑100) | BW25113_RS01680/BW25113_RS01685 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/3); new base A (0/9); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGG > NZ_CP009273/340935‑341100 | ttCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGc < 2:279573/90‑1 (MQ=255) atGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTaa < 1:191263/90‑1 (MQ=255) aaaaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGc > 1:103841/1‑90 (MQ=255) tGTTTGTGGGGGGTTTTTTCTGTATCTTACGCACCGCCCTCAACCCTGAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTa < 2:27204/90‑1 (MQ=255) gTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaa > 1:116116/1‑78 (MQ=255) gTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaa < 2:116116/78‑1 (MQ=255) gTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAg > 1:27204/1‑90 (MQ=255) ttGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCACATCAAAAACCGCCATTAGCt > 2:149503/1‑90 (MQ=255) ttGTGGGGGTTTTTTTCTTTATCTTCCCCACCCCCCCCAAGCCTAAAAAAAAATATGCTGAAAGGCTCATAAAAAAAACCGCCATTAGCt < 1:109076/90‑1 (MQ=255) ttGTGGGGGGTTTTTTCTGTATCTTCCGCATCGCACTAAACCCTGAAAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCt < 2:214037/90‑1 (MQ=255) ttGTGGGGGGTTTTTTCTGTATCTTACGCATCCCACTCAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAACCCGCCATTAGCt < 2:310126/90‑1 (MQ=255) tgtgtgGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCtc > 1:328912/1‑90 (MQ=255) tgGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCtcatca > 2:105552/1‑90 (MQ=255) gggTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAgg > 2:305289/1‑90 (MQ=255) ggTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGa > 1:281594/1‑90 (MQ=255) aTCTTACGCATCGCACTCAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac < 2:54622/90‑1 (MQ=255) ttACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAc > 2:44887/1‑90 (MQ=255) ttACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAc > 2:49409/1‑90 (MQ=255) cATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGa > 1:274861/1‑90 (MQ=255) tCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt > 1:324291/1‑90 (MQ=255) tCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt > 2:144764/1‑90 (MQ=255) ccTCAAGCCTGAAAAAAAATATGTTGTAGGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg < 2:182818/89‑1 (MQ=255) aCTCAAGCCTAAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg < 2:31056/90‑1 (MQ=255) aaGCCTAAAAAAAAATATGCTGAAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATg < 1:78705/90‑1 (MQ=255) gACAAAAAATGTGCTGTAAGGCTCATAAAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACgg < 1:170124/90‑1 (MQ=255) | TTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGG > NZ_CP009273/340935‑341100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |