Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273371,0310TG61.5% 2.7 / 10.3 13G39G (GGT→GGG) mhpT3‑(3‑hydroxy‑phenyl)propionate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (8/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCG‑CCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGG  >  NZ_CP009273/370946‑371090
                                                                                     |                                                            
cccGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCa                                                          <  2:289785/90‑1 (MQ=255)
  cGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCgggggggggc                                                        >  1:285453/1‑86 (MQ=255)
       gATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCG‑CCCa                                                  <  2:23496/90‑1 (MQ=255)
             gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGGTCG‑CCCAGGCgtt                                            >  2:63209/1‑90 (MQ=255)
             gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCG‑CCCCGGCgtt                                            >  1:191571/1‑90 (MQ=255)
                    gggCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCG‑CCCAGGCTTTCGCACTc                                     <  1:325255/90‑1 (MQ=255)
                         ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCAt                                                         <  2:20984/66‑1 (MQ=255)
                         ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGAGCG‑CCCCGGCTTTTCCACTCCATaa                                >  1:57661/1‑90 (MQ=255)
                         ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGCAt                                                         >  1:20984/1‑66 (MQ=255)
                            ttttttGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCG‑CCCAGGCTTTCGCACTCGATAAAAt                             <  2:234535/90‑1 (MQ=255)
                                     cGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGCATCG‑CCCCGGGTTTTGCACTCGATAAAAAGCAAATggg                    >  2:172627/1‑90 (MQ=255)
                                                 aGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGGTCG‑CCCCGGCTTTTGCACTCGATAAAATGCAAATGGGGTGGGTATTTAg        >  1:124530/1‑90 (MQ=255)
                                                 aGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGGTAGCCCCGGGCTTTC‑CCCCCGGTAAAAAGCAAAAGGGGGGGGTAttttt        >  1:175922/1‑88 (MQ=255)
                                                       ggATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCG‑CCCCGGCTTTTGCCCTCGCTAAAAAGCAAAAGGGGTGGGTATTTTTCGCCgg  >  1:317047/1‑90 (MQ=255)
                                                                                     |                                                            
CCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCG‑CCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGG  >  NZ_CP009273/370946‑371090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑