Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,794,5080TG54.5% 4.2 / 10.7 11V62G (GTA→GGA) nrdIclass Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (6/0);  total (8/3)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGAGGCGTATG  >  NZ_CP009273/2794429‑2794594
                                                                               |                                                                                      
aTTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGGACGGCTGGCGCGGGGGCACCGCAGGGAAATTGCttt                                                                              >  1:31689/1‑90 (MQ=255)
        aGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGGAATTCGCTTTTTAAacga                                                                      >  1:50796/1‑90 (MQ=255)
                        cTGATCGTGCCCTCTTACGGCGGCGGCGGGACGGCTGGCGCGGGGCCCCGGCAGGGAATTTCCTTTTTAAAAGACGGGCCCAAACggggg                                                      >  2:209870/1‑88 (MQ=255)
                              gTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCtt                                                >  1:329771/1‑90 (MQ=255)
                                gCCCTCTTACGGCGGCGGCGGGACGGGTGGCGGGGTGCCCCGACAGGGGATTTGCGTTTTTAAAGACGGGCCCAAACGGGCGGTGCGTcg                                              >  2:165557/1‑90 (MQ=255)
                                             ggcggcggGACGGCTGGGGCGGGGCCCCGACAGGGAATTTGCTTTTTTAAACACGAGCACAACCGGGGGGTGCTTCGCGGCGGTATTTCt                                 >  2:347102/1‑90 (MQ=255)
                                                            ggCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGc                                                                 <  1:156014/43‑1 (MQ=255)
                                                            ggCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGc                                                                 >  2:156014/1‑43 (MQ=255)
                                                                   tGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTg           <  2:267036/90‑1 (MQ=255)
                                                                    gCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGa          <  1:167543/90‑1 (MQ=255)
                                                                            aGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGAGGCGTATg  >  2:87327/1‑90 (MQ=255)
                                                                               |                                                                                      
ATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGAGGCGTATG  >  NZ_CP009273/2794429‑2794594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑