| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,485,389 | 0 | G | T | 55.6% | 5.4 / 13.3 | 9 | Q90K (CAG→AAG) | ppiA | peptidylprolyl isomerase A |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base T (5/0); total (5/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGT > NZ_CP009273/3485310‑3485457 | cGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGc < 2:260318/90‑1 (MQ=255) tGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTTCTGCATCTTCTCGGTGGAAacgg > 2:257210/1‑89 (MQ=255) tgtTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGGTTTTTT‑TTCTGCATCTGCTCGGGGGAAACGCCCCCCCGAAtta > 2:129465/1‑88 (MQ=255) gcAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGGTGGGTTTTTTTTCTGCCTCTGCTCGGGGGAAACCCCCCCCCCAAACATaaaaa > 2:184884/1‑88 (MQ=255) ttATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATg < 1:129465/90‑1 (MQ=255) ttATCGGCTTCATTTTTGATTGGC‑GGGGTTTGTTTTTTTTTCTGCATCTTCTCGGGGGAAACCCCCCCCCGAATCATAAAAACAGGGATg > 1:171476/1‑90 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 1:262250/90‑1 (MQ=255) gATTGGCGGGGTTTGGTTTTTT‑TTCTGCCTCTTCTCGGTGGAAACCCCCCCCCGAATTATAAAAACAGGGAAGAGGCGGGGGAAGGGAgt > 2:106852/1‑90 (MQ=255) ttGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtgt < 2:171476/90‑1 (MQ=255) | CGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGT > NZ_CP009273/3485310‑3485457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |