| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,142,237 | 0 | T | G | 53.3% | 7.6 / 12.9 | 16 | T264P (ACC→CCC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGACAGTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACC‑GGAACAAATTCGACGGG > NZ_CP009273/4142152‑4142318 | aTCGACAGTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGata < 2:74681/90‑1 (MQ=255) cGACAGTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATc > 1:211485/1‑90 (MQ=255) cAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGAA‑GTTCgg > 2:301333/1‑90 (MQ=255) gCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGAC‑GGTCgggg > 1:183205/1‑89 (MQ=255) tttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTc < 1:55260/90‑1 (MQ=255) tttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGAG‑GTTTGGGGTGGCCTCGCgggg > 2:154436/1‑88 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGCGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTcgcc > 1:149278/1‑90 (MQ=255) ggAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATACCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTcgccgc < 2:208870/90‑1 (MQ=255) aaCAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGGCGGTTGGGGTTG‑CGGCGCGGGGGCCGccc > 2:314493/1‑90 (MQ=255) tCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCAc < 2:106358/90‑1 (MQ=255) tCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCAc < 2:211485/90‑1 (MQ=255) gCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGGTATCGGCAGGGGC‑GGTCGGGGTGGCGGCGCGGGCGCCGCCCCCCCCCGAAgga > 2:887/1‑88 (MQ=255) cAGGACGTGGCGGGTGATATCGGCAGGGAC‑GTTCGGGTTGCCGGCGCGGGCGCCGCCCCTCCACGAAGGAAAAAGGACC‑GGGAAAAAATc > 1:280186/1‑90 (MQ=255) aGGACGTGGCGGGGGATATCGGCAGTGAC‑GTTCGGGGTGCCGTCGCGGGCGCCCCCCCTCCCCGAAGGAAAAAGGGCCGGGAAAAAAttg > 2:2232/1‑89 (MQ=255) gTGGCGGGTGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACC‑GGAACAAATTCGACggg < 1:314493/90‑1 (MQ=255) gTGGCGGGGGATATCGGCAGTGGC‑GTTCGGGGTGGCGGCGCGGGTGCCGCCCCTCCACGAAAGAAAAAGGACC‑GGGACAAAATCGACggg > 2:112385/1‑90 (MQ=255) | ATCGACAGTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGAC‑GTTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACC‑GGAACAAATTCGACGGG > NZ_CP009273/4142152‑4142318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |