Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,326,7180TA53.8% 8.0 / 16.8 13Y44F (TAT→TTT) adiCarginine/agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base A (0/7);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 5.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACAC  >  NZ_CP009273/4326650‑4326794
                                                                    |                                                                            
cGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTag                                                         >  2:103750/1‑90 (MQ=255)
cGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTag                                                         >  2:4626/1‑90 (MQ=255)
                   gTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTaa                                      >  2:147493/1‑90 (MQ=255)
                   gTATACCATCGAGAGCCCCAGTGCACCGATAAACGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTaa                                      >  2:153503/1‑90 (MQ=255)
                             gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAaca                            >  1:195020/1‑90 (MQ=255)
                                 gCCCCAGTGCACCGATAATCGTCCCCAACCACCAAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg                        <  1:136286/90‑1 (MQ=255)
                                 cccccAGTGCACCGATAACCGCCCCCACCCACCAAAAAAGGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTg                        <  2:239291/89‑1 (MQ=255)
                                   cccGGTGCCCCAATAATCGTCCCCACCCATCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAc                      <  1:228294/90‑1 (MQ=255)
                                     cggTGCACCAATAATCGCCACCACCCACCCAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAccc                    <  1:296342/88‑1 (MQ=255)
                                        tGCACCAATAATCGTCACCACCCACCCAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCat                 <  1:25727/90‑1 (MQ=255)
                                            ccGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataata             >  2:306230/1‑90 (MQ=255)
                                             caaaaaTCGTCCCCACCCACCCAAAAATGCAAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAatat            <  2:195020/86‑1 (MQ=255)
                                                       ccccacccacCCAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGacac  <  2:144225/88‑1 (MQ=255)
                                                                    |                                                                            
CGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACAC  >  NZ_CP009273/4326650‑4326794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑