| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,910 | T→A | W30R (TGG→AGG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,910 | 0 | T | A | 100.0% | 84.5 / NA | 25 | W30R (TGG→AGG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (13/12); total (13/12) | |||||||||||
CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49828‑49991 | cAGCCTGATTGCGGCGTTAGCGGTAGCTCGCGTTATCGGCATGAAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCAGGTTTaa < 2:213728/90‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTaaa > 1:213269/1‑90 (MQ=255) gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAAc < 2:244308/90‑1 (MQ=255) cTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGc > 1:25835/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCaa < 1:98942/90‑1 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCt > 1:107562/1‑90 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCt < 1:124858/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGGTTAAACGCAACACCtt > 1:253015/1‑90 (MQ=255) aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGt < 2:150461/90‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCCTGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:54675/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGGGATTa > 2:299178/1‑90 (MQ=255) aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt > 2:188988/1‑90 (MQ=255) cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc < 1:50854/76‑1 (MQ=255) cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc > 2:50854/1‑76 (MQ=255) aTGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTtaaataaa > 1:216933/1‑57 (MQ=255) aTGCCGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTtaaataaa < 2:216933/57‑1 (MQ=255) ccGTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc < 2:213269/90‑1 (MQ=255) gTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCtt < 1:90386/45‑1 (MQ=255) gTGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAACACCtt > 2:90386/1‑45 (MQ=255) tGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAac > 2:100589/1‑39 (MQ=38) tGGAACCTGCCTGCCGATCTCGCCAGGTTTAAACGCAac < 1:100589/39‑1 (MQ=38) cctgcctgCCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc < 2:107562/90‑1 (MQ=255) tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt > 1:127815/1‑90 (MQ=255) tgcctgccGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt > 1:199710/1‑90 (MQ=255) aTCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc < 1:91529/90‑1 (MQ=255) | CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49828‑49991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |