Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,303,883	A→C	V215V (GTT→GTG)	pnp ←	polyribonucleotide nucleotidyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,303,883	0	A	C	100.0%	23.2 / NA	8	V215V (GTT→GTG)	pnp	polyribonucleotide nucleotidyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (4/4);  total (4/4)

GCTTCGTTTACCGGCTCCGGCTGCCAGTCCCAACGCGGTTTACCGGCTTCTTTCACCAGTTCATTGATGTTCTGAATAACAACCTGCTGTTGTTCATGACCGAACACTACTGCGCCCAGCATCTGGTCTTCGCTCAGCAGTTGAGCTTCAGATTCAACCATC  >  NZ_CP009273/3303806‑3303967                                                                              |                                                                                     gcTTCGTTTACCGGCTCCGGCTGCCAGTCCCAACGCGGTTTACCGGCTTCTTTCACCAGTTCATTGATGTTCTGAATCACAACCTGCtgt                                                                          <  1:159142/90‑1 (MQ=255) gcTTCGTTTACCGGCTCCGGCTGCCAGTCCCAACGCGGTTTACCGGCTTCTTTCACCAGTTCATTGATGTTCTGAATCACAACCTGCtgt                                                                          <  1:337696/90‑1 (MQ=255)           ccggctccggctGCCAGTCCCAACGCGGTTTACCGGCTTCTTTCACCAGTTCATTGATGTTCTGAATCACAACCTGCTGTTGTTCATGAc                                                                <  1:50318/90‑1 (MQ=255)                  cggctGCCAGTCCCAACGCGGTTTACCGGCTTCTTTCACCAGTTCATTGATGTTCTGAATCACAACCTGCTGTTGTTCATGACCGAacac                                                         >  1:33806/1‑90 (MQ=255)                                                           gTTCATTGATGTTCTGAATCACAACCTGCTGTTGTTCATGACCGAACACTACTGCGCCCAGCATCTGGTCTTCGCTCAGCAGTTGAGCtt                >  2:137548/1‑90 (MQ=255)                                                                    tGTTCTGAATCACAACCTGCTGTTGTTCATGACCGAACACTACTGCGCCCAGCATCTGGTCTTCGCTCAGCAGTTGAGCTTCAGATTCaa       >  1:142880/1‑90 (MQ=255)                                                                     gTTCTGAATCACAACCTGCTGTTGTTCATGACCGAACACTACTGCGCCCAGCATCTGGTCTTCGCTCAGCAGTTGAGCTTCAGATTCAAc      >  2:135761/1‑90 (MQ=255)                                                                         tGAATCACAACCTGCTGTTGTTCATGACCGAACACTACTGCGCCCAGCATCTGGTCTTCGCTCAGCAGTTGAGCTTCAGATTCAACCATc  <  2:148942/90‑1 (MQ=255)                                                                              |                                                                                     GCTTCGTTTACCGGCTCCGGCTGCCAGTCCCAACGCGGTTTACCGGCTTCTTTCACCAGTTCATTGATGTTCTGAATAACAACCTGCTGTTGTTCATGACCGAACACTACTGCGCCCAGCATCTGGTCTTCGCTCAGCAGTTGAGCTTCAGATTCAACCATC  >  NZ_CP009273/3303806‑3303967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑