Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,390,316	A→C	L280R (CTG→CGG)	rng ←	ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,390,316	0	A	C	100.0%	19.6 / NA	7	L280R (CTG→CGG)	rng	ribonuclease G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/5);  total (2/5)

CTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTC  >  NZ_CP009273/3390230‑3390396                                                                                       |                                                                                 cTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCcgcg                                                                               >  1:89138/1‑90 (MQ=255)                       ggtCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCCGCGCTCGCTGGATTTCGTTTTCGAc                                                         <  2:120487/90‑1 (MQ=255)                                      cTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCCGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGaa                                          >  2:46981/1‑90 (MQ=255)                                             aTAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCCGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGc                                   <  1:245900/90‑1 (MQ=255)                                                  gagaTAACCACCGGATTTCAGTTCTACTTTGCGTTCCCGCGTTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCg                              <  2:219237/90‑1 (MQ=255)                                                     aTAACCACCGGATTTCAGTTCTACTTTGCGTTCCCGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTcc                           <  1:46981/90‑1 (MQ=255)                                                                   tCAGTTCTACTTTGCGTTCCCGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCa             >  1:193420/1‑90 (MQ=255)                                                                              ttGCGTTCCCGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTc  <  1:42508/90‑1 (MQ=255)                                                                                       |                                                                                 CTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTCCAGCTTGCTTGTC  >  NZ_CP009273/3390230‑3390396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑