Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,527,045	A→C	S123A (TCG→GCG)	nanS ←	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,527,045	0	A	C	100.0%	84.4 / NA	25	S123A (TCG→GCG)	nanS	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/14);  total (11/14)

GCGGAGTATCCGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGATCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATAT  >  NZ_CP009273/4526958‑4527119                                                                                        |                                                                           gCGGAGTATCCGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTc                                                                          >  2:304923/1‑90 (MQ=255)      gTATCCGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCa                                                                     >  2:181011/1‑90 (MQ=255)         tCCGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACgg                                                                  <  2:326079/90‑1 (MQ=255)           cGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCa                                                                >  2:260810/1‑90 (MQ=255)           cGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCa                                                                >  2:197388/1‑90 (MQ=255)               ccccAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAacac                                                            <  1:236952/90‑1 (MQ=255)                  cAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGa                                                         >  2:58235/1‑90 (MQ=255)                  cAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGa                                                         <  2:273609/90‑1 (MQ=255)                   aaCGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGaa                                                        >  2:324648/1‑90 (MQ=255)                           gCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGa                                                <  1:109892/90‑1 (MQ=255)                                 tggctggcTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCt                                          <  1:87366/90‑1 (MQ=255)                                         tCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATc                                  <  1:181011/90‑1 (MQ=255)                                         tCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATc                                  <  1:61979/90‑1 (MQ=255)                                                 gTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGa                          <  1:214371/90‑1 (MQ=255)                                                           tGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTaa                >  2:144476/1‑90 (MQ=255)                                                           tGTCCCTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTaa                >  2:225455/1‑90 (MQ=255)                                                                cTTCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTcc           >  1:98415/1‑90 (MQ=255)                                                                 ttCGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCg          <  1:199331/90‑1 (MQ=255)                                                                   cGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTg        <  1:197388/90‑1 (MQ=255)                                                                   cGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTg        <  1:133072/90‑1 (MQ=255)                                                                   cGCTGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTg        <  2:98415/90‑1 (MQ=255)                                                                      tGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCaa     <  2:203366/90‑1 (MQ=255)                                                                      tGCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCaa     >  1:105608/1‑90 (MQ=255)                                                                       gCCCGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAat    <  1:324648/90‑1 (MQ=255)                                                                         ccGCGGTAAAAGCCGCTCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAatat  >  2:156683/1‑90 (MQ=255)                                                                                        |                                                                           GCGGAGTATCCGTTCCCCAACGACAAGCATCATGGCTGGCTCCGTGCCGTTCTGAATATGTCCCTTCGCTGCCCGCGGTAAAAGCCGATCCGCCACGGCAACACGGAACGATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATAT  >  NZ_CP009273/4526958‑4527119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑