| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,123,179 | 0 | G | T | 63.6% | 1.2 / 13.0 | 11 | L212F (TTG→TTT) | metF | methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (7/0); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTC > NZ_CP009273/4123097‑4123267 | tcttcGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTTCGGGGATTTTTCCGGGAt > 1:10967/1‑90 (MQ=255) aCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAAATATTCCGGGGATTTTTCCGGGATCTAACTTTTAAAAAGCgg > 1:106812/1‑89 (MQ=255) cTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGGATTTTTCCGGGATCTAACTTTTAAAAGGGGAAGAAAATTTCCGATATGAc > 2:102706/1‑90 (MQ=255) ggcggGCATTGATGTGGGAATTATTCCGGGAATTTTTCCGGGATCTAACTTTAAAAAGGGGAAGAAATTTGCCGATATGACCAACGTGCg > 1:83303/1‑90 (MQ=255) ggcggGCATTGATGTGGAAATTATTCCGGGGATTTTTCCGGGATCTAACTTTAAACAGGCGAAGAAAATTGCCGATATGACCAACGTGCg > 1:102327/1‑90 (MQ=255) gggCCTTGATGTGGGAAATATTCCGGGAAATTTTGCGGTATCTAACTTTAAACAGGCGAAGAAAATTTACGATATTTCCAACGTGCGGAt > 2:82666/1‑90 (MQ=255) gggCATTGATGTGGAAATTATTTCGGGAATTTTTCCGGTATCTAACTTTAAAAAGGCGAAGAAATTTGCCGATATGACCAACGTGCGGAt > 2:236531/1‑90 (MQ=255) ggAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGAt > 1:93859/1‑90 (MQ=255) aTTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGAc < 2:83303/90‑1 (MQ=255) ttttGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACg > 2:17549/1‑90 (MQ=255) tGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTc < 2:102327/90‑1 (MQ=255) | TCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTC > NZ_CP009273/4123097‑4123267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |