| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,544,923 | 0 | C | A | 55.6% | 1.6 / 11.6 | 9 | N177K (AAC→AAA) | uxuR | Uxu operon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base A (5/0); total (5/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCAC > NZ_CP009273/4544839‑4544995 | gCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGaaaaaaaac > 2:160213/1‑87 (MQ=255) gTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCCaa > 2:262897/1‑90 (MQ=255) ccATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAAtgtg < 2:101953/90‑1 (MQ=255) aTCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAAAAAACCAATGTggg > 1:64153/1‑89 (MQ=255) gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAAACAAAGTGGATCCAg > 2:67907/1‑90 (MQ=255) aCGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAACCAAAGTGGGTCCAGTTTCACAGCCCTCTg > 1:230270/1‑90 (MQ=255) ttCCGTCAGTCCTGGCAGTGGC‑GGGGAAAAAAAACAATATGGGTCCAGTTGCCCAGCCCTCTGGGTGGCAGCCCTGATTGCAAAGAgggg > 1:135110/1‑90 (MQ=255) cAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGc < 1:195882/90‑1 (MQ=255) cAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGc < 1:253627/90‑1 (MQ=255) tGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATcac < 1:203965/90‑1 (MQ=255) | GCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGC‑GGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCAC > NZ_CP009273/4544839‑4544995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |