| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,341,237 | 0 | T | G | 58.8% | 11.1 / 14.9 | 17 | V255G (GTG→GGG) | rapZ | RNase adapter RapZ |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (10/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCA > NZ_CP009273/3341154‑3341325 | gCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTatat < 2:172570/90‑1 (MQ=255) aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTGc > 1:85745/1‑90 (MQ=255) tACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTGCAGAGc > 1:65507/1‑90 (MQ=255) aTGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTTCAGAGCAACTg > 1:57394/1‑90 (MQ=255) aTGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCCCTCGGGGGATATTGCAGAGCAACTg > 2:17275/1‑90 (MQ=255) gCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGc < 2:45459/90‑1 (MQ=255) gTAGATACTTGCCGGTCGCCCTGGGGTGTGCCGGCGGGCAGCACCGTTCGGTGTATATTGCAGAGCACCTGGCCCAATTCCTCCGCTcgc < 2:224291/90‑1 (MQ=255) tGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTaaaa < 1:40456/90‑1 (MQ=255) tGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTaaaa < 1:36760/90‑1 (MQ=255) gTCGCCATTGGTTGTACCGGCGGGAAGAACCGGTCGGGGTATATTTCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGGAAAAAAGTc > 1:194561/1‑90 (MQ=255) cGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCa < 2:237913/90‑1 (MQ=255) tACCGGCGGGAAGGACCGTTCGGGGGATATTGCAAAGCAACTGGCAGACTACTTCCCCCCGCGCGGGGAAAAAGGCCCGGCACGCCCTcc > 2:173517/1‑89 (MQ=255) tACCGGCGGGAAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACCACTTCCGCTCGCGCGGGAAAAACGTCCAGTCACGCCCTCg > 1:77189/1‑90 (MQ=255) tACCGGCGGGAAGCACCCGTCGGGGTATATTTCAGAGCAACTGGCAGACTACTTCCCCCCCCGCGGGAAAAAAGTCCAGTCACGCCCTCg > 2:3777/1‑90 (MQ=255) ggcggGAAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGGAAAAAAGTCCAGTCACGCCATCGTACg > 2:219302/1‑90 (MQ=255) gcggGAAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACTACTTCCGCCCGCGCGGGAAAAAAGTCCCGTCACGCCACCGTACGc > 2:223853/1‑90 (MQ=255) aGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaa < 2:52930/90‑1 (MQ=255) gtgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCa > 1:282330/1‑90 (MQ=255) | GCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCA > NZ_CP009273/3341154‑3341325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |