| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,168,592 | 0 | A | C | 54.5% | 7.7 / 11.4 | 11 | V55G (GTG→GGG) | parE | DNA topoisomerase IV subunit B |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAC > NZ_CP009273/3168510‑3168681 | ctctTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGttt > 1:104993/1‑90 (MQ=255)ctctTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGttt > 1:117077/1‑90 (MQ=255) cccGGCACCCCCCCCCCATCGTCAATACCTTCTAACGACGGGTCAGCATGAAAAATACCGCCCCCGCTTTTTGCGTGCCCCGCCAGTGCt < 2:286286/89‑1 (MQ=255) ccccccGCCCCCATCGTCAATACCTTCTAACGCCGGGTAAGCATGAAAAATACCGCCCCCGTTTTTTGCGTGCCCCGCCAGTGCTTCATc < 2:232968/87‑1 (MQ=255) aCTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGActtct > 1:324688/1‑90 (MQ=255) gccTGGTCAGAATGAAAAATACCCCCCCCGCTTTTTGCGGGCCCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCaaa < 2:198940/88‑1 (MQ=255) ggTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGt > 1:9251/1‑90 (MQ=255) aTGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCg > 2:230296/1‑90 (MQ=255) tgaaaaaTACCGCCCCCGCTTTTGGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGa < 2:2710/87‑1 (MQ=255) aaaaaTACCGTCCCCGCTTTTTGCGTGCCCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGt < 2:324688/89‑1 (MQ=255) aaaaaTAACGTCCCCGCTTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGt < 1:144476/89‑1 (MQ=255) aCGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAc > 1:5987/1‑90 (MQ=255) | CTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTATAC > NZ_CP009273/3168510‑3168681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |