| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,234,344 | 0 | T | G | 60.0% | 2.9 / 20.2 | 20 | S348A (TCT→GCT) | sstT | serine/threonine transporter SstT |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (12/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGC > NZ_CP009273/3234264‑3234433 | cGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTc‑tgctgc > 2:61826/1‑90 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTc‑tgctgctg > 1:163784/1‑90 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTc‑tgctgctg > 1:93370/1‑90 (MQ=255) gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTc‑tgctgctg > 1:63820/1‑90 (MQ=255) cGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGAt < 2:9818/90‑1 (MQ=255) cGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTC‑TGCTGCTGAt > 1:330778/1‑90 (MQ=255) cGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCCc‑tgctgctgtg > 1:59049/1‑88 (MQ=255) tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCAc < 2:208675/90‑1 (MQ=255) tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCAc < 2:330778/90‑1 (MQ=255) ccACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCACTgg < 1:143502/90‑1 (MQ=255) aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCACTGGcc < 2:187105/90‑1 (MQ=255) ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTC‑TGCTGCTGATCCCACTGGCCTGTAat > 1:21151/1‑90 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTC‑TGCTGCTGATCCCCCCGGCCTGTAATATGTTTggggtt > 2:119923/1‑86 (MQ=255) gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTC‑TGCTGCTGATCCCCCCGGCCCGTAATATGTTTGGGAtt > 2:157760/1‑90 (MQ=255) gCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAAc < 2:93370/90‑1 (MQ=255) ctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTTGCGGTTGTTCCCCCTGGCCTGAAAAATTTTGGGATTTTCGAACGaatt > 1:94909/1‑87 (MQ=255) gtgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc < 2:251979/90‑1 (MQ=255) ccTGTGGCGCATCCGGCGTGGCGGGGGGGGCTC‑TTCTGCTGATCCCCCTGGCCCGTAATATGTTTGGGGTTTCGGACGGTATCGCCACGc > 2:245984/1‑90 (MQ=255) aTCCGGCGTGGCGGGGGGGGCTC‑TGCTGCTGATCCCCCCGGCCCGTAATATGTTTGGGGTTTTGAACGATATCGCCATGCAGGGGGGTGc > 2:344060/1‑90 (MQ=255) ggggggTCTC‑TGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc < 1:327034/90‑1 (MQ=255) tctc‑TGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGCTGCCGTCGGCTTTATCATCggt > 1:191467/1‑89 (MQ=255) | CGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTC‑TGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGC > NZ_CP009273/3234264‑3234433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |