| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,698,302 | 0 | A | C | 54.5% | 8.8 / 11.2 | 11 | G88G (GGT→GGG) | ydgJ | oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGG > NZ_CP009273/1698213‑1698379 | agCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTa > 1:295716/1‑90 (MQ=255)agCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTa > 2:255842/1‑90 (MQ=255) gCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGATAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCt > 2:103632/1‑90 (MQ=255) cAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTCATCAACGACCACATGTTTACCCGCCTCAAg > 1:345141/1‑90 (MQ=255) gCTTTTCCCCACCGCACCCCCCTCCCCCCCTTGGGCCCGTGCCACGGTAATGGT‑TTCATCACCGCCCCCATTTTTCCCCGCCTCAAgcgc < 1:106263/90‑1 (MQ=255) tctcGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATgg > 2:298121/1‑90 (MQ=255) tgtgACGGTGTCAGGGAAAGGGT‑TTTAACAACGCCCAATTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 1:221446/90‑1 (MQ=255) tgtgACGGTG‑CAAGGGAAAGGGTTTTACCAACGCCCACATTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 2:41300/90‑1 (MQ=255) aGTGTCAGGGAAAGGGT‑TTTACCACCGCCCAATTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTa < 1:37221/90‑1 (MQ=255) aaaGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAAt > 2:100248/1‑90 (MQ=255) gttttATCAACGACCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAgg < 1:255842/88‑1 (MQ=255) gACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg > 1:200289/1‑90 (MQ=255) ccccACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg < 2:59232/88‑1 (MQ=255) | AGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGG > NZ_CP009273/1698213‑1698379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |