Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	32.2 / NA	11	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (3/8);  total (3/8)

GATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA  >  NZ_CP009273/49834‑49969                                                                             |                                                            gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                <  1:299435/90‑1 (MQ=255)  aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                               <  1:194634/90‑1 (MQ=255)      cggcgTTGGCGGTAGATCGCGTTATCGGCTTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                           <  2:2886/90‑1 (MQ=255)      cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                           <  2:20855/90‑1 (MQ=255)      cggcgTTAGCGGTAGACCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                           <  2:55561/90‑1 (MQ=255)       ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                <  1:30169/84‑1 (MQ=255)       ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                >  2:30169/1‑84 (MQ=255)             aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaataa                                    <  2:106378/90‑1 (MQ=255)                   aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                              <  1:123360/90‑1 (MQ=255)                    gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTg                             >  2:59505/1‑90 (MQ=255)                                               aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca  >  1:148186/1‑90 (MQ=255)                                                                             |                                                            GATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA  >  NZ_CP009273/49834‑49969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑