Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,170,8900AC54.5% 4.2 / 11.5 11V127G (GTG→GGG) nudFADP‑ribose diphosphatase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCAC  >  NZ_CP009273/3170807‑3170957
                                                                                   |                                                                   
cGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGtt                                                               >  1:143423/1‑90 (MQ=255)
   ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                            <  2:31094/90‑1 (MQ=255)
            tGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGa                                                   <  1:56432/90‑1 (MQ=255)
                gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTAt                                               >  1:102149/1‑90 (MQ=255)
                                  aaTTGACGACCCCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAAt                             <  1:119416/90‑1 (MQ=255)
                                           aCGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgcg                    >  2:54913/1‑90 (MQ=255)
                                            cccTCCCTGGTCCCCCCCGGGCTGGCAAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa                   <  2:143423/88‑1 (MQ=255)
                                                   tGGTCCCCCCCGGGCTGGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCaa            <  1:37329/90‑1 (MQ=255)
                                                             ggggTTTGCCAGGAAACTAACCCCCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCcac  <  2:174568/89‑1 (MQ=255)
                                                             cGGGTTTGCCAGAAAATTTAACCCCGTTTTGGCCCTTTTGCCTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCcac  <  1:182915/90‑1 (MQ=255)
                                                             cGGGCTTGCCAGAAACCTAACCCCCGTTTTGGCCCTTTTGACTATCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCcac  <  2:62301/90‑1 (MQ=255)
                                                                                   |                                                                   
CGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCAC  >  NZ_CP009273/3170807‑3170957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑