| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,744,897 | 0 | A | C | 61.5% | 0.6 / 11.2 | 13 | V111G (GTT→GGT) | yiaT | MipA/OmpV family protein |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/8); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGG > NZ_CP009273/3744818‑3744979 | ccaGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCAt > 2:212450/1‑90 (MQ=255) caGCCGTTGCTGTTATCCAGACCCCCCCCAGCTGCAGAGGTTTTCACGGTCCCCCAACGCTCGGGGTGTACCCAGGCCCCCCCCGCCATa < 2:191797/90‑1 (MQ=255) gCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGc > 1:106362/1‑90 (MQ=255) gttgttgttACCCAGACCGCCCGCAGCTGCAGAGGTTTCCCCGGTTCCCCAACGCTGGTGGTGATACCGGCCACCCCCCGCCATAGCCGt < 2:41537/90‑1 (MQ=255) tCCAGACCGCCCGCAGCTGCAGAGGTTTTCACGGTCCCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGc < 2:106362/90‑1 (MQ=255) cgcccGCAGCGGCAGGGCCTTCCACGGTCCCCCACCCCTGGGGGTAAACCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCaa < 2:223073/87‑1 (MQ=255) cGCAGCGGCAGAGCCTTCCTCGGTCCCCCACCCCTGGTGGTGATCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTg < 2:230367/90‑1 (MQ=255) gCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAccc > 1:50114/1‑49 (MQ=255) gCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAccc < 2:50114/49‑1 (MQ=255) ccACGTTCCCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGt < 1:212450/89‑1 (MQ=255) gtcccccAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTa < 1:37737/87‑1 (MQ=255) gCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGt > 2:84750/1‑90 (MQ=255) ccAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCgg < 1:77075/90‑1 (MQ=255) | CCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGG > NZ_CP009273/3744818‑3744979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |