| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 297,088 | 0 | A | C | 57.1% | 10.5 / 14.8 | 14 | L171V (TTG→GTG) | paoB | aldehyde oxidoreductase FAD‑binding subunit PaoB |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAA > NZ_CP009273/297008‑297177 | cGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGc > 2:159431/1‑90 (MQ=255) gAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGa > 1:49355/1‑90 (MQ=255) tCACCCGGTGTAATCCTGCGTTTCTTCCCCCCGGGGGGGAGGTTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTc < 2:28228/90‑1 (MQ=255) gtgATACTGCGAGTTTTCCCCCCGGGGGTGGTGTTTCCCCCCCCCGCACCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTg < 2:245151/90‑1 (MQ=255) gCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCa > 2:134143/1‑90 (MQ=255) gTCTTCCCCTCGGGCTTGTTGGTTCCCCCCCCCGCCCCCAACACCGGTTTTGCCACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCt < 2:98977/90‑1 (MQ=255) gTCTTCCCCTCCGGCGTGATGGTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCt < 1:86461/90‑1 (MQ=255) tttCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCg > 2:58004/1‑90 (MQ=255) cccccGGGGTGTTGTTTCCCCCCCCCGCACCCAGCACCCGCTTGGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 2:73453/87‑1 (MQ=255) cccccGGCGTGATGGTTCCCCCCCCCGCATCAAGCACCCGCTTTGCGCCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 2:199364/87‑1 (MQ=255) ccTCCGGCGGGATGTTTCCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 1:159431/90‑1 (MQ=255) ccgggCGGGATGACTCCCACCACCGCAACCGGCACCCCCTTTGCCCCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACg < 2:186625/87‑1 (MQ=255) ggCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCt > 2:70204/1‑90 (MQ=255) aTCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTaaa > 2:31469/1‑90 (MQ=255) aCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCaa > 1:226894/1‑90 (MQ=255) aCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGGGCTGACGGCTAAAGCCTTCaa > 2:90444/1‑90 (MQ=255) | CGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAA > NZ_CP009273/297008‑297177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |