| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,940 | 0 | T | A | 57.1% | ‑1.1 / 14.9 | 14 | T63S (ACC→TCC) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base A (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGA > NZ_CP009273/4058865‑4059019 | gACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGcca < 2:45925/90‑1 (MQ=255) aCGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGccac > 1:245834/1‑90 (MQ=255) cGAAATTTGCCTTTAGGCCCAATATTTTTCCGCAAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGccacc < 2:33376/90‑1 (MQ=255) caaaTTTTTTCCCGCGAGTCGAAAAAAAATCCGGTGACCATATCGGAAAACGCGGAAAAAACCTTCGCCACCAGAAAGAAACCCCCGCCa < 1:679/88‑1 (MQ=255) ccGCGAGTGGAGAAAAAACCCGGTGAGCATATCGGAAAACGCGGAAAAAAACTTCCCCACAAAAAAGAAAATCCCGCCATAGTAAGCAgg < 2:257011/90‑1 (MQ=255) ccGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAgg > 1:256010/1‑90 (MQ=255) ccGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAgg > 2:161405/1‑90 (MQ=255) cgcgAGTCGAAAAAAAACCGGGTGAACATACCGAAAAACGCGAAAAAAACCTCCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGc < 1:234342/90‑1 (MQ=255) gcgAGTGGAAAAAAAACCCGGTGACAATATGGGAAAACGGGGAAAAAAACTCCCCCCCCAAAAAAAAAACCCCGCCATAGTAAGCAGGCa < 1:161405/90‑1 (MQ=255) gcgAGTCAAGAAAAAATCCGGTGAGCATATCGGAAAACGCGGAAAAAACCTCCGCCACAAGAAAGATAACCCCGCCATAGTAAGCAGGCa < 1:3483/90‑1 (MQ=255) gCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt > 1:97028/1‑90 (MQ=255) taccGGAAAACGCGAAAAAAAACTCCGCCCCAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATCCATCGGTATAAAATTTga < 2:30804/87‑1 (MQ=255) tCGGAAAAGGCGGAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:1583/90‑1 (MQ=255) gaaaaCGCGAAAAAAACCTTCCCCACAAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 2:256010/88‑1 (MQ=255) | GACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGA > NZ_CP009273/4058865‑4059019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |