| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,440,600 | 0 | T | G | 52.0% | 15.9 / 26.2 | 25 | G274G (GGT→GGG) | ytfQ | galactofuranose ABC transporter substrate‑binding protein YtfQ |
| Reads supporting (aligned to +/- strand): ref base T (4/8); new base G (13/0); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACC > NZ_CP009273/4440523‑4440682 | ggTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAc < 1:129552/90‑1 (MQ=255) tgtACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCGAcgc > 1:99114/1‑90 (MQ=255) tgtACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCTTCGAcgc > 2:79484/1‑90 (MQ=255) gtACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCGAcgcg > 2:294320/1‑90 (MQ=255) aCCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCt < 2:288416/90‑1 (MQ=255) ggACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGTCCGCCTTCGACGCGCTggg > 1:212425/1‑89 (MQ=255) cATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCGACGCGCTGGGGaa > 1:73851/1‑90 (MQ=255) cATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCCTCGCCGCGCTGGGGaa > 1:298515/1‑90 (MQ=255) aTCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGGCCGCCCTCGGCGCGCCGGGGaaa > 1:188376/1‑90 (MQ=255) tCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAAt > 2:113387/1‑90 (MQ=255) tCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCTTCGACGCGCTGGGGaaaa > 2:293878/1‑89 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTcc < 1:214661/61‑1 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGGCGCGCTGGAGAAATaaaaaa > 2:71169/1‑90 (MQ=255) aaGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGcc > 2:214661/1‑61 (MQ=255) gCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCCACGCGCTGGGGGAATACaaaaaa > 1:194994/1‑90 (MQ=255) gatgatGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCCCCTTCGACGCGCTGGAGAAATACAAAAAAGa > 2:219243/1‑90 (MQ=255) tgatgGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACg < 1:113387/90‑1 (MQ=255) tggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCGACGCGCTGGGGAAAAACAAAAAAAACGGCACCAt > 1:53161/1‑90 (MQ=255) aaCTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCa < 2:256900/90‑1 (MQ=255) gACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGGGAAAAACAAAAAAAACGGCACCATGCCTGAAAAGCTGACGTTAAACAAATc > 1:113310/1‑90 (MQ=255) gACGCCGAATATGGCAGGGCCCCCCCTCGCCGCGCTGGGGAAAAAAAAAAAAAACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc > 1:291118/1‑90 (MQ=255) aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATcc < 2:53161/90‑1 (MQ=255) cGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc < 1:294320/90‑1 (MQ=255) cGAATAGGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAAACGGCACCATGCCTGAAAAACTGACGTTAACCAAAACCAccc > 2:262179/1‑90 (MQ=255) tGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTAcc < 1:79484/90‑1 (MQ=255) | GGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACC > NZ_CP009273/4440523‑4440682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |