| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,464,532 | 0 | T | G | 52.9% | 10.2 / 12.4 | 17 | *198G (TGA→GGA) | bdcR | TetR/AcrR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base G (9/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAT > NZ_CP009273/4464453‑4464618 | aCGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGcc > 2:53749/1‑90 (MQ=255) agagGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTg < 2:138882/90‑1 (MQ=255) aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGaaa < 2:98231/90‑1 (MQ=255) aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGaaa > 2:174790/1‑90 (MQ=255) acaacTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGaaaa > 1:17030/1‑89 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGGGGGATGCGGGGCTTGACCCGAAAGGCGGGGAAacgc > 1:285537/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGATGCGGGGCTTGACCCGAAAGGCGGGGAAacgc > 1:306072/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGGGGAGGGATGCGGGCCTTGACCCCAAAAGGGGGaaaaggc > 1:42197/1‑86 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 2:77949/1‑90 (MQ=255) acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAgcgc > 2:105923/1‑90 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGGAAGGATAAAAAAAATTTTTTGGGAGGGATGCGGGGCTTGACCCGAAAGGGGGGGAAACGCCTGcc > 1:151552/1‑90 (MQ=255) gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGcc > 2:87611/1‑90 (MQ=255) cGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAACGCCCGCCGATAAGGTGTGATAAGAc > 1:230862/1‑90 (MQ=255) gATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAc < 1:174790/90‑1 (MQ=255) aTTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGc > 2:228818/1‑90 (MQ=255) tGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCg > 1:165324/1‑90 (MQ=255) gagTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAt < 1:77949/90‑1 (MQ=255) | ACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAT > NZ_CP009273/4464453‑4464618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |