| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 15,410 | 0 | T | G | 53.3% | 9.8 / 14.9 | 15 | intergenic (+112/‑35) | dnaJ/BW25113_RS00080 | molecular chaperone DnaJ/IS4‑like element IS421 family transposase |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGC > NZ_CP009273/15321‑15473 | ggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGt < 1:294636/90‑1 (MQ=255) aGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgt < 2:250311/90‑1 (MQ=255) aGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgt < 2:56738/90‑1 (MQ=255) ggCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg < 1:229426/90‑1 (MQ=255) gTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAc < 2:107891/90‑1 (MQ=255) aGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTAAGGGGTGTGGGATTACGCCTGgta > 2:140892/1‑90 (MQ=37) aGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata < 1:189145/90‑1 (MQ=255) gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACTCCCCTAAACGCTAAATTTAAGGGTGTGGGGTTTTGCCTGatat > 1:107891/1‑90 (MQ=255) gTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTTACTCCCCTAAACGCTAACTTTAAGGGTGTGGGGTTTCGCCTGGTATGa > 2:55419/1‑90 (MQ=255) tatGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCCAAACGCCAAATTAAAGGGTGGGGGATTTAGCCTGATATGATTTTAAGTGGCg > 1:71937/1‑90 (MQ=255) tGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCCAAACGCGAACTTTAGGGTTGTGGGATTACGCCCGGTATGATTTTAAGTGGCGAt > 2:336160/1‑90 (MQ=255) gAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTAAAGGGTGTGGGATTACGCCCGATATGATTTTAAGTGCCGGTGaa > 2:265867/1‑90 (MQ=255) gAGCACCTGTAAAGTGGCGGGGGTCACTCCCCCAAGCGCTAACTTAAGGGGTGGGGGGTTTTGCCTGATATGATTTTACGTGCCGCTGaa > 1:44520/1‑90 (MQ=18) gAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAGCGCTAAATTAAAGGGTGTGGGGTTAAGCCTGGTATTATTTTAAGTGCCGATGaa > 1:262746/1‑90 (MQ=255) ggCGGGGGTCACTCCCCCAAACGCTAACTTAAGGGGTGGGGGGTTTCGCCCGATATGATTTTACATGCCGGGGAATTAATCTCCCCATaa > 1:143839/1‑90 (MQ=9) cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGc < 1:336160/90‑1 (MQ=33) | GGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGC > NZ_CP009273/15321‑15473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |