Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,090,8260TG53.8% 9.1 / 12.7 13G67G (GGT→GGG) hemWradical SAM family heme chaperone HemW
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (7/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCA  >  NZ_CP009273/3090748‑3090898
                                                                              |                                                                        
gCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGGGccc                                                               >  2:253844/1‑89 (MQ=255)
              aTCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTccggcccgg                                                 >  2:131077/1‑90 (MQ=255)
               tCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTccggcccggc                                                <  2:257844/90‑1 (MQ=255)
                cTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGGGCCCGCTTTTCCGCCCcgcg                                               >  1:108950/1‑90 (MQ=255)
                      aaCGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCaa                                         <  1:80995/90‑1 (MQ=255)
                        cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAc                                       <  1:145058/90‑1 (MQ=255)
                            gtgGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACgctg                                   <  2:315573/90‑1 (MQ=255)
                            gtgGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGGCCGCTTTTCGGCCCCGCGGGGCAAAAgctg                                   >  1:114303/1‑90 (MQ=255)
                                  tACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGAc                             <  1:244086/90‑1 (MQ=255)
                                   aCGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGCCCGCTTTTCGGCCCGGCGGTGCAAAACCTGCTggggg                            >  1:78681/1‑87 (MQ=255)
                                        cAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCTTTCCCGCCCCGCGATGCAAACCCCGCTGGgcggcggg                       >  2:209260/1‑88 (MQ=255)
                                                            aaTCTTTATTGGCGGTGGGGAACCAAACCTCCTTTCCAGCCCGGCGAAGAAAACGCTCCCGGGCGGGGGGCGGGCGCGGTTGCCCCTGGc   >  2:200108/1‑90 (MQ=255)
                                                             aTCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGGTTGGCGCTGGcc  >  2:270176/1‑89 (MQ=255)
                                                                              |                                                                        
GCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCA  >  NZ_CP009273/3090748‑3090898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑