| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,502,009 | 0 | C | G | 56.2% | 9.9 / 19.7 | 16 | A388A (GCG→GCC) | yhfW | phosphopentomutase |
| Reads supporting (aligned to +/- strand): ref base C (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTG > NZ_CP009273/3501926‑3502092 | gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGC‑gg > 2:139358/1‑90 (MQ=255) gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGC‑GGgaaaaat > 2:207715/1‑88 (MQ=255) tCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAAtt > 2:2224/1‑90 (MQ=255) aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTcacac < 2:95928/90‑1 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAAATAACACACCGGAGcc > 2:158007/1‑90 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGC‑GGGAAAAATAACACACAGGAGcc > 1:106393/1‑90 (MQ=255) ggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGC‑GGGAAAAATTACACAACGGAGccccccc > 1:50246/1‑88 (MQ=255) gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGC‑GGGAAAAAACACACACCGGAGCCCCccca > 1:167782/1‑90 (MQ=255) gtCTCCTGCAAACCGGAGGGAGGAAAAAAAAGAGCGACCATTTTGTGGCGGGGGGGCGC‑GGGAAAAATTACACACGGGAGCCCCcccacc > 1:311257/1‑88 (MQ=255) tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGGGC‑GGGAAAAATAAAACACCGTAACCCCCGCACCAAAAAg > 1:166258/1‑90 (MQ=255) cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCg < 1:224419/90‑1 (MQ=255) aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtg < 1:341165/90‑1 (MQ=255) aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtg < 2:335935/90‑1 (MQ=255) aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCacac < 2:167782/90‑1 (MQ=255) agaaaagaGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTTACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc < 1:225745/90‑1 (MQ=255) agCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg < 2:50246/90‑1 (MQ=255) cggtggcgCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTg < 1:150950/90‑1 (MQ=255) | GTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTG > NZ_CP009273/3501926‑3502092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |