| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,695,567 | 0 | A | C | 60.0% | 5.9 / 12.1 | 15 | T118T (ACA→ACC) | malY | bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGT > NZ_CP009273/1695491‑1695655 | gCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACg > 2:402689/1‑90 (MQ=255) cTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCAt > 1:229231/1‑90 (MQ=255) tcgtCTATATGTTTTAAAAACAGATTAGTCGGTGGTCGGAAACAGGAAACGGGGTGGTGACCCCCCCCCCCGCCTATGACGCATTTTACa < 2:348984/87‑1 (MQ=255) tCATTTATATGTTTTCAGAACTGATTCGTCAGTGGTCTAAAACGGGTGAAGGCGTGGTGACCCACACCCCCGCCTATGACGCATTTTACa < 2:457245/90‑1 (MQ=255) cATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACaa > 1:114596/1‑90 (MQ=255) ttCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAgg > 1:482207/1‑90 (MQ=255) tCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGt > 1:228320/1‑90 (MQ=255) tcgtcgGTGGTCTGAAACGGGTGAGGGCGTGGTGACCCACCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGcac < 1:402689/90‑1 (MQ=255) tCGTTAGTGGTCTAAAAAAGGTGAGGGGGGGGTGACCCCCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGcac < 2:436042/90‑1 (MQ=255) tCGTCAGTGGTCTAAAACAGGTGAAGGCGTGGTGACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGcac < 2:23603/90‑1 (MQ=255) tcgaaaaCAGGTAAGGGGGTGGTGACCCCCACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGccc < 2:294005/86‑1 (MQ=255) tCTAAAACAGGTGAGGGCGTGGTGACCCCCCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGccc < 1:294809/90‑1 (MQ=255) taaaaCGGGTGAAGGCGGGGTGACCCACCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGt < 2:88215/88‑1 (MQ=255) gAAACGGGTGAAGGCGTGGTGACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGtt < 2:228320/90‑1 (MQ=255) aaaaCGGGTGAGGGGGGGGTGACCCCCACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGtt < 1:51448/89‑1 (MQ=255) cacCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGAtggttggt > 1:176923/1‑90 (MQ=255) | GCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGT > NZ_CP009273/1695491‑1695655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |