| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,769,914 | 0 | A | G | 52.9% | 7.3 / 16.5 | 17 | E57G (GAG→GGG) | yfjY | RadC family protein |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base G (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTA > NZ_CP009273/2769840‑2769991 | gcgcGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGCGGGGAGAAttttt > 2:407116/1‑88 (MQ=255) aaaCAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGCGTGAAGAATTTATTATGc > 2:195541/1‑90 (MQ=255) aCAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGGTGGGGCGGGAAGAAATTTTTATGCTg > 2:269857/1‑90 (MQ=255) ggCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTAt > 2:107970/1‑90 (MQ=255) ggCATTCACCTCCGCTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGGTGGGGGGGGGAGAAATTATGATGCTGTATCTGaa > 1:269579/1‑90 (MQ=255) ggCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGaa < 2:462186/90‑1 (MQ=255) cAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGa > 1:38928/1‑82 (MQ=255) cAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGa < 2:38928/82‑1 (MQ=255) ggCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGGTGGGGGGGGGAGAATTTTTTATGCTGTATTTGAACCAGCAGAAACAGGTGAt > 1:268819/1‑90 (MQ=255) ggCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGAt < 2:146696/90‑1 (MQ=255) ggCTGAAGCTGAAAATGGCGGGGGTGGGGGGGGGAAAAATTTTTATTGTGTTTTTTAAACAGCAGAAACAGGTTATTTGCCACGAAAacc > 1:310778/1‑90 (MQ=255) ggCTGAAGCTGAAAATGGCGGGGCTGGGGCGGGGAGAATTTTTTATGCTGTATCTGAACCAGCAGAACCAGGTGATTGCCCCCCAAAacc > 2:296056/1‑90 (MQ=25) aagctgaaAATGGCGGGGCTGGGGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAAACCTGttt > 1:14538/1‑90 (MQ=255) ctgaaAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGcc < 2:209068/90‑1 (MQ=255) gaaAATGGCGGGGCTGGGGGGTGAAGAAATTTTTATGCTGTATCTGAAACAGCAGAACCAGTTGGTTGGCCACGAAAACCTGTTTGCCgg > 1:302768/1‑90 (MQ=255) aaaTGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGtt < 1:246490/90‑1 (MQ=255) tGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTATATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTa < 1:46370/90‑1 (MQ=255) | GCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTA > NZ_CP009273/2769840‑2769991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |