| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,805 | 0 | T | G | 58.8% | 12.3 / 17.6 | 17 | V785G (GTT→GGT) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (10/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTA > NZ_CP009273/2852725‑2852882 | tATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGGTATTAAgcg > 1:173840/1‑90 (MQ=255) cacaGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCa < 1:201492/90‑1 (MQ=255) cacaGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTCGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCa < 2:464030/90‑1 (MQ=255) acaGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGGAGGGGGGGCAAAAAAGGGTATTAAacgcgccc > 1:422562/1‑88 (MQ=255) acaGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAACGCGCAc > 2:355461/1‑90 (MQ=255) acaGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCCCTGGCAGGCGGGCCAAAAGAGGGTATTAAACGCGCAc > 1:194735/1‑90 (MQ=255) caGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCCGCTCCGGCAGGCGGGCCAAAAAAGGGTATTAAACCCGCACg > 1:460684/1‑90 (MQ=255) gTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGGGGGCCAAAAAAGGGTATTAAGGCCGCACGGGaa > 1:231948/1‑90 (MQ=255) tGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGGTATTAAGCGCGCACGGCaaa > 1:21089/1‑90 (MQ=255) cAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGGTATTAAGCGCGCACGGCAAAAg > 1:397544/1‑90 (MQ=255) gcgcGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCCAAAGAGGGTATTAAGCGCGCACGGCAAAAACTGCGTg > 1:213250/1‑90 (MQ=255) cggcgAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGc < 1:298021/90‑1 (MQ=255) ttGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAACGCGCCCGGCAAAAGCTGCGGGGGCTGGAAAGCATTTTGCCGAAcgccgccg > 1:460005/1‑90 (MQ=255) gCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCt < 2:213250/90‑1 (MQ=255) gCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCt < 2:360740/90‑1 (MQ=255) gCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATg > 2:448575/1‑90 (MQ=255) tGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTa < 2:269117/90‑1 (MQ=255) | TATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTA > NZ_CP009273/2852725‑2852882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |