| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,969 | 0 | C | T | 52.6% | 6.6 / 17.5 | 19 | L21F (CTC→TTC) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base C (2/7); new base T (10/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAAT > NZ_CP009273/3175900‑3176057 | gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGTTTTTCTGCGTTCTCGGG‑Caaaaa > 2:313053/1‑90 (MQ=255)gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCCCGGTTTTTTGGGCGGTTTTCGGCGGTCTCTGG‑Ccaaaa > 2:446833/1‑90 (MQ=255) aGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACCGTTTTTGGGGGGGTTTTCCGCGGTCTCCGG‑Caaaaaa > 2:223020/1‑89 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGCGCGGTTTTTCGCGGTCTCCGG‑Cgaaaaac > 1:59871/1‑88 (MQ=255) aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAAc < 1:223020/90‑1 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGCGGCCCCCTTTTTTGGCTCGTTTTTCCGCGTTTTTCGG‑GAAAAACCCCCCAACCCCttt > 1:215496/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCCGCGTTCTCGGG‑CAAAAAACCCCCAACCGCCTTCTggg > 2:302367/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGGTTTTCGGCGTTTTTGGGCCAAAAACCCCCAAACC‑CTTTCTGGc > 1:119505/1‑90 (MQ=255) ctcATTCTGACCATACTGGCGGGGGGCGCCCCGTTTTTTTGCGCGTTTTTCCCCGTTCTCGGG‑CCAAAAACCCCGAACCGCTTTCTGGCg > 1:211132/1‑90 (MQ=255) cATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGtt < 1:438302/90‑1 (MQ=255) aTTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGttt < 1:66153/90‑1 (MQ=255) tGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGGGCGGTTTTCCGCGTTCTCCGG‑CAAAAAACCCCCAACCCCCTACTTGCGgtttttc > 1:317368/1‑87 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 1:434434/90‑1 (MQ=255) aTACTGGCGGGGGCCGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGCTTTCCCTAGGt > 2:184577/1‑90 (MQ=255) aTACTGGCGGGGGCAGCCACGTTTATTGGCGCGGTTTTCGGCGTTCTCGGG‑CAAAAACCCCCGAACCGCTTACTGGCGGTTTTGCTAggg > 2:66153/1‑89 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:215496/90‑1 (MQ=255) ggCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatc < 1:319010/90‑1 (MQ=255) cgcgTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatctc > 2:36662/1‑90 (MQ=255) cgcgTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatctc < 1:313053/90‑1 (MQ=255) tctcGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAAt < 2:211132/90‑1 (MQ=255) | GAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG‑CAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAAT > NZ_CP009273/3175900‑3176057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |