| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,357,755 | 0 | A | T | 48.0% | 17.7 / 13.5 | 25 | P530P (CCA→CCT) | yhcD | fimbrial biogenesis outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (10/3); new base T (0/12); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTC > NZ_CP009273/3357673‑3357836 | ccGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCa < 2:200106/90‑1 (MQ=255) gcagcaTCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTT‑GGCAGATCAAacac < 1:68268/90‑1 (MQ=255) gcaTCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTTTTCTCCGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGc < 1:3139/90‑1 (MQ=255) tCAACTTAGTGCTTAAAAAAATAATGAAGACGATGGTGATCTTTTCTCCCTCAACATCAGTATTCCTTTT‑GGCAGATCAAACACCGCcac < 1:351737/90‑1 (MQ=255) gTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGtata > 2:360078/1‑90 (MQ=255) gTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGtata > 1:353441/1‑90 (MQ=255) gTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGtata > 2:258144/1‑90 (MQ=255) tGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTTTACGTAAACATCAGTTTTCCTTTT‑GGCAGATAAAACCCCGCCACACTGTATAc < 2:484718/90‑1 (MQ=255) tGCTTCAAAAAAAAATAAAGACGAGGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTT‑GGAAGACAAAACACCGCCACACTGTATAc < 1:8143/90‑1 (MQ=255) ataatGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACg < 2:72473/90‑1 (MQ=255) taatGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGa > 2:142612/1‑90 (MQ=255) aatAAAGAGGGTGGTAATCTTTTCTACGCCAACATCA‑TTTTCCATTTGGGAAGACAAAACACCGCCACACTGTATACTCGCCATGACGAt < 1:101135/90‑1 (MQ=255) aTGGTGATCTTTTCTACGTAAACACCATTTTTCCTTTT‑GGCAAAACAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAAccc < 1:360078/90‑1 (MQ=255) gtaaTCTGTTCTACGTAAACATAAGTATTCCTTTT‑GGAAAACAAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACt < 2:433427/87‑1 (MQ=255) tttCTACGCAAAAACCATTTTTCCTTTT‑GGCAGATAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTAc < 2:375131/89‑1 (MQ=255) gTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTAc > 2:49554/1‑90 (MQ=255) ttCTACGTAAACATCAGTATTCTTTTT‑GGAAAACAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACt < 1:354879/90‑1 (MQ=255) tCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTg > 2:189123/1‑90 (MQ=255) tCTACGAAAACATCAGTTTTCCTTTT‑GGAAAATAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTg < 1:209250/90‑1 (MQ=255) aCGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgtg > 2:456432/1‑90 (MQ=255) aaCATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATg > 2:83423/1‑90 (MQ=255) tCAGTATCCTTTTT‑GGAAAACAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAg < 1:142612/90‑1 (MQ=255) cAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgt > 2:50254/1‑90 (MQ=255) aGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtc > 2:292682/1‑90 (MQ=255) aGTATCCCTTTT‑GGCAGATAAACCACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtc < 2:281103/90‑1 (MQ=255) | CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTT‑GGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTC > NZ_CP009273/3357673‑3357836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |