| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,694 | 0 | T | C | 57.1% | 3.8 / 12.0 | 14 | P177P (CCT→CCC) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base C (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCG > NZ_CP009273/3738605‑3738762 | ccAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCt < 2:389633/90‑1 (MQ=255) aaTTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCCgg > 1:66395/1‑90 (MQ=255) tCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTTTCCCCGGCCTGTTTATgggg > 1:367885/1‑89 (MQ=255) aTTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCa < 2:133267/90‑1 (MQ=255) gTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCGTTGCCCCCGGCGTGATGAGGGGGGGGACGCt > 1:357166/1‑90 (MQ=255) gTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGGGCAACGCt > 2:200467/1‑90 (MQ=255) ttatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGc < 1:300539/90‑1 (MQ=255) tatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCt < 1:33832/90‑1 (MQ=255) ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTTCCCCCGGCATGATGATGGGCGCAACGCTGATGCTTTcc > 1:455830/1‑90 (MQ=255) tATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTc < 2:69955/90‑1 (MQ=255) agtCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCt < 1:256262/88‑1 (MQ=255) aaGCTGTTTATGGCGGGGATTTCCCCCGGGATGATGGTGGGGGCAACGCTGATGATTACCTGGGGGGGGGAGGCCCGGCGCCTTTAtttt > 2:320625/1‑89 (MQ=255) aaGCTGTTTATGGCGGGCATTTCCCCCGGCATGATGATGGGGGCAAAGCTGATGCTTACCTGGGGGGGGGAGGGCCGGCGCCTTTAtttt > 1:302521/1‑89 (MQ=255) aaGCTGTTTATGGCGGGCATTGCCCCCGGCCTGATGATGGGGGCAACGCTGATGCTTACCTGGGGGGGGGAGGCCCGTCGCCTTTAtttt > 1:452810/1‑89 (MQ=255) gTTTATGGCGGGCATTGCCCCCGGCCTGGTTATGGGGGCAACGCCGTTGCTTTCCCGGGGGGGGGAGGCCCGTCGCCTTTATTTTcccct > 2:256262/1‑89 (MQ=255) | CCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCG > NZ_CP009273/3738605‑3738762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |