| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,102,362 | 0 | T | A | 57.1% | 8.0 / 17.1 | 14 | N40K (AAT→AAA) | yiiR | DUF805 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base A (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGC > NZ_CP009273/4102278‑4102449 | gCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACt < 1:447859/90‑1 (MQ=255)gCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAAATACt > 2:447859/1‑90 (MQ=255) gTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCg < 1:357597/90‑1 (MQ=255) gTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAAACTACTcc > 2:472412/1‑89 (MQ=255) ccGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAATTACTCGATATTTAGAc > 2:357597/1‑90 (MQ=255) ccGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAACTACTCGGTATTctaac > 1:219638/1‑86 (MQ=255) aTTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCgcgg < 1:470810/90‑1 (MQ=255) gATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTtgt < 1:365798/90‑1 (MQ=255) gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAAATACTCGATATTTAGAACGCGGCGGTTTGCCCTTTGtgctttct > 2:254685/1‑90 (MQ=255) gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAAAAACTAATCCATATTTAGACCCCGGCGGTTTTCCTTTTGtgcttgct > 2:267684/1‑90 (MQ=255) tCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAACTACTCGATATTTAGACCGCGGCGTTTTTCCTTTTGTGCTTGCTCTGGc > 1:350295/1‑90 (MQ=255) aCTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAgcg < 1:384121/90‑1 (MQ=255) cTGGCGGGGAAGAAACTACTCGATATTCAGACCGCGGCGGTTTTCCTTTTGTGGTTTCTCTGGCCAACAACGGGCGGAAATGTTaaaggc > 1:192193/1‑86 (MQ=255) aaTCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATcgc > 2:71927/1‑90 (MQ=255) | GCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGC > NZ_CP009273/4102278‑4102449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |