| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,290,979 | 0 | T | G | 64.3% | 4.1 / 10.9 | 14 | T639T (ACA→ACC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (9/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACC > NZ_CP009273/4290891‑4291065 | gggCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg < 1:67660/90‑1 (MQ=255) gTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAg > 2:195503/1‑90 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTTTCTTCAAGCGGTa > 2:284650/1‑90 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGGTa > 2:43845/1‑90 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTTAAACGttt > 2:433611/1‑89 (MQ=255) taataaGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGc < 1:284650/90‑1 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCCAGCGTTATTTTAGGGGAAAGGCTGAGGGGGGGTGCCCGACCGGCAGCGGAttt > 2:345953/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTAGGAGAACGGCTGAGGGCGGGTTCCCGACCGGCAGCGGAttt > 2:101666/1‑90 (MQ=255) gggCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTGAGGGGAAAGGCTGAGGGCGGGTGCCCGACCGGCCGCGTATTtttc > 2:240634/1‑90 (MQ=255) tGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGt < 1:428885/90‑1 (MQ=255) tGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTAGGGGAACGGCCGAGGGGGGGTGCCCGACCCGCCGCGGATTTTTTCAgggggg > 1:338333/1‑87 (MQ=255) agagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCaaa < 1:101666/90‑1 (MQ=255) agagGCGGGGGTCTCTTCAAGCGTTATTTTAGGAGAACGGCTGGGTGGGGGTGCCAGACCGGCAGCGTATTTTTCCCGGGGGGCGGCaaa > 1:150382/1‑90 (MQ=255) gggTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGAcc < 2:150382/90‑1 (MQ=255) gggTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGAcc < 2:460703/90‑1 (MQ=255) | GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACC > NZ_CP009273/4290891‑4291065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |