| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 349,999 | 0 | T | G | 53.8% | 0.6 / 12.4 | 13 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC > NZ_CP009273/349916‑350084 | tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGTGGAAGa > 1:117782/1‑90 (MQ=255) tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAaggg > 2:111406/1‑89 (MQ=255) ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGGGGGGGGGAAGAAGGGGTGGAAggggg > 1:361490/1‑88 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:127575/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:33509/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCGGTTTTCAt > 2:350911/1‑90 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCCGAAAGGGGAGGGGGGGGGGGGGGTTGGCAt > 1:19271/1‑90 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAAAGGGGCTGGAAAGGGGGGGGGCGGGGGGGTTTTTCAATCCGa > 2:486239/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTTATTCCGAAagag > 2:268155/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 1:111406/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGAAAGGGGAGGGGGGGGGGGGGGTTGTTATTTCGGAAGAGAGCggc > 1:267091/1‑88 (MQ=255) gggCGTTGCCGAAGTGGCGGTGGTTGGGTTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg < 2:117782/90‑1 (MQ=255) ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 2:47165/90‑1 (MQ=255) | TGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC > NZ_CP009273/349916‑350084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |