Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,723	G→C	G116A (GGC→GCC)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,723	0	G	C	100.0%	33.3 / NA	12	G116A (GGC→GCC)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (7/5);  total (7/5)

GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAA  >  NZ_CP009273/1613637‑1613795                                                                                       |                                                                         gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCa                                                                       <  2:205504/90‑1 (MQ=255)                    cgcgGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTa                                                    <  1:474137/90‑1 (MQ=255)                                  aaaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAc                                      >  1:111276/1‑90 (MQ=255)                                    aaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcgg                                    <  1:151055/90‑1 (MQ=255)                                       ttACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGAcc                                                                  <  1:362834/57‑1 (MQ=255)                                       ttACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGAcc                                                                  >  2:362834/1‑57 (MQ=255)                                                  ggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCa                      <  1:388162/90‑1 (MQ=255)                                                           atatGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAg             >  1:228405/1‑90 (MQ=255)                                                               gtgAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt         >  1:384648/1‑90 (MQ=255)                                                                      aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa  >  2:295440/1‑90 (MQ=255)                                                                      aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa  >  2:4020/1‑90 (MQ=255)                                                                      aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa  >  2:7156/1‑90 (MQ=255)                                                                                       |                                                                         GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAA  >  NZ_CP009273/1613637‑1613795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑