Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273602,0530AG54.5% 0.5 / 11.9 11W263R (TGG→CGG) ybdKYbdK family carboxylate‑amine ligase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base G (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAA  >  NZ_CP009273/601976‑602127
                                                                             |                                                                          
aCAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGc                                                                >  2:499769/1‑90 (MQ=255)
                          aCAGCAGGTATTTTTTTTCTTGTTTTTTAACGGGGCGTTCCGTCAGAAACCGGGGGGGGGTAGCCTAAATTAACCCCGCCATATTTACTg                                      <  2:177983/90‑1 (MQ=255)
                            agcagTTATTTTTTTTCTTGTTTTTAAAGGGGGCTTTCCGTCAGAACCGGGGGGGGGGTAGCTTAAATAACCCCCGCCATATTTACTGCg                                    <  1:70896/90‑1 (MQ=255)
                                  tAATTTTTTTCCTGATTTTAAAGGGGGCTTCCCGTCAGTACCCGGGGGGGGGTAGCCTAAATTAATCCCGCCATATTTACTGCGTGGCTa                              <  2:222647/90‑1 (MQ=255)
                                        ttttCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTa                                                        <  1:542535/58‑1 (MQ=255)
                                        ttttCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTa                                                        >  2:542535/1‑58 (MQ=255)
                                                 tGTTAAAGCGGGCTTTCCGTAAGAACCCGGGGGGGGGTAGCCTAAATAACCCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTa               <  1:187415/90‑1 (MQ=255)
                                                           ggCGTCCCGTCAGTACCCGGGGGGGGGTACCATAAATTAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCAccc     <  2:278332/90‑1 (MQ=255)
                                                           ggCGTCCCGTCAGAACCGGGGGGGGGGTACCTTAAATTAACCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCAccc     <  2:520774/90‑1 (MQ=255)
                                                            gCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCg    >  1:434309/1‑90 (MQ=255)
                                                              gTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGaa  >  2:385956/1‑90 (MQ=255)
                                                                             |                                                                          
ACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATTAATCCCGCCATATTTACTGCGTGGCTAAGGGTTAACGGGGTATCCATCACCCGAA  >  NZ_CP009273/601976‑602127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑