| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,564,395 | 0 | T | G | 54.5% | 16.0 / 24.5 | 22 | N239T (AAC→ACC) | eutE | aldehyde dehydrogenase EutE |
| Reads supporting (aligned to +/- strand): ref base T (3/7); new base G (12/0); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTGTTATCGAAAGAAGCGCCTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCA > NZ_CP009273/2564312‑2564479 | tgttgtTATCTAAAGAAGCGCCTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGCTTCATCCACCCCCACCCGCGGGGTGGCaa > 1:83242/1‑89 (MQ=255) tgttATCGAAAGAAGCGCCTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAgcgc > 2:136088/1‑90 (MQ=255) aTCGAAAGAAGCGCCTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGc < 2:220763/90‑1 (MQ=255) agCGCCTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGGCCGCGCCCGCGGCAATCAg > 1:161039/1‑90 (MQ=255) cTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTTCCCACCCCCGCGGCAAACAGAC‑ggt > 2:352254/1‑88 (MQ=255) tGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCCGCGGGGTGCCAGCGCCCGCGGCAAACAGGCGGTTTTTTgggg > 2:324590/1‑88 (MQ=255) cTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGCCCGCGCCCGCGGCGAACAGAC‑GGTTTTTTGGGTGttt > 1:119897/1‑90 (MQ=255) gAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGGCCGCGCCCGCGGGGAACCGAC‑GGTTTTTTGTGTGgttta > 1:277205/1‑88 (MQ=255) gAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGCCCGCGCCCGCGGCAATCAGAC‑GGTTTTTTGTGGGttttc > 1:109112/1‑88 (MQ=255) gAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGCCCGCGCCCGCGGCAATCAGAC‑GGTTTTTTGGGTGttttc > 1:142061/1‑88 (MQ=255) gAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGCCAGCGCCTGCGGCAATCAGAC‑GGTTTTTTGGGGGTTTAc > 2:354227/1‑90 (MQ=255) ggCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACgcgc < 2:277205/90‑1 (MQ=255) ggCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACgcgc < 1:136088/90‑1 (MQ=255) gCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGcgcc < 2:468453/90‑1 (MQ=255) gCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGGCAGGGCCCGCGGGAAACAGAC‑GGTTATTGGGGGGGTTTCGcgcc > 2:534146/1‑90 (MQ=255) aCGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGCCAGCGCCTGCGGCAATCAGAC‑GTTTTTTGGGGGGTTTTCGcgccgc > 2:394506/1‑90 (MQ=255) cGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGCGCCGCt < 1:302093/90‑1 (MQ=255) ggCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGGTGCCAGCGCCTGCGGCAATCAGAC‑GGTTTTTTGTGGGTTTTCGCGCCGCTTc > 1:43604/1‑90 (MQ=255) ggTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTTTTGGTGTGTTTACGCGCCGCTTctacta > 2:50007/1‑90 (MQ=255) gcggTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCt < 1:202447/90‑1 (MQ=255) gcggGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACcagca < 1:352254/90‑1 (MQ=255) gcggGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACcagca > 1:40126/1‑90 (MQ=255) | TGTTGTTATCGAAAGAAGCGCCTTTGACGATGGACTGAGCGGCACGGGCGAGGTCGGCGGTTTCATCCACCACTACCGGCGGGTTGCCAGCGCCTGCGGCAATCAGAC‑GTTTATTGGTGTGTTTACGCGCCGCTTCTACTACCGCTTCGCCGCCGGTTACCACCAGCA > NZ_CP009273/2564312‑2564479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |