Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,363,6730GT61.5% ‑2.1 / 15.3 13L13I (CTT→ATT) nanKN‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (0/8);  total (4/9)
Fisher's exact test for biased strand distribution p-value = 6.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTC  >  NZ_CP009273/3363609‑3363756
                                                                |                                                                                   
gctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGcc                                                            <  2:100445/90‑1 (MQ=255)
 ctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCa                                                           >  2:370770/1‑90 (MQ=255)
             gTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAg                                               >  2:8363/1‑90 (MQ=255)
                     cgaTCGGGGTTCTGCCGGTCAGCGCCAATCGGCGGGGGGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCt                                       <  1:107274/90‑1 (MQ=255)
                          gcgGTTCTGCCCGTCAGCGCCAACCGGCGGGGCGGCAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTc                                  <  1:370770/90‑1 (MQ=255)
                               tCTGCCCGTCAGCCCCAATCAGCGGGGCGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGc                             <  2:270665/90‑1 (MQ=255)
                                  gCCCGTCAGCGCCAATCGGCGCGGCGCCATTTTTAGTTCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCtgt                          <  1:417307/90‑1 (MQ=255)
                                  gCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCtgt                          >  1:232843/1‑90 (MQ=255)
                                         ggcgcCATTCTGCGCGGGGGAAATTTTTGCGCCGCCGATATCAATCGCCAGTGGGGTCATAGCTCCGCCTTTTTCATCGCTGTGTTGTAc                   <  1:82360/89‑1 (MQ=255)
                                           cgcAAATCGGCGGGGGGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCa                 <  2:503113/90‑1 (MQ=255)
                                               aaTCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGa             >  2:127053/1‑90 (MQ=255)
                                                   ggcggGGCGGAAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACaaa         <  1:480481/89‑1 (MQ=255)
                                                          cggcAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTc  <  1:65315/90‑1 (MQ=255)
                                                                |                                                                                   
GCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTC  >  NZ_CP009273/3363609‑3363756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑