| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,703,332 | 0 | A | C | 57.1% | 7.6 / 11.7 | 14 | G168G (GGT→GGG) | eptB | kdo(2)‑lipid A phosphoethanolamine 7''‑transferase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGT > NZ_CP009273/3703245‑3703414 | gCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATAcc > 1:347953/1‑90 (MQ=255) cGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTac > 2:344068/1‑90 (MQ=255) aaaCCACCGCCGTCCGCCCCCCCCCCTTTCTTCTGCTGAATACCCACCAAACGAATCGGTGCCCAAACCAAAATCCCCGCCAGTacgacg < 2:64731/90‑1 (MQ=255) cGCCGGCCCCCCTCCCCCCTTTCTTCTGCTGGATACCCAGAAAACGAATCGGTCCCCAAACCATAATCCCCGCCAGTACGACGACCGCCa < 2:496819/90‑1 (MQ=255) ccctcTTTCTTCTGCGGGATACCCAGCAAACGAATCGGTCCCCAACCCATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGc < 2:148280/86‑1 (MQ=255) cccccTTTCTTCTGCTGAATACCCAGCAAACGAATCGGTCCCAAAACCATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGc < 2:224756/86‑1 (MQ=255) ttctGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTggg > 2:397265/1‑90 (MQ=255) tgcggGATACCCAGCAAACAAATCGGTCCCAAAACAATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGtt < 2:193647/90‑1 (MQ=255) gctgAATACCCAAAAAACAAATGGGCCCCAAAACAAAAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTc < 2:289232/90‑1 (MQ=255) ctgGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCg > 1:289232/1‑90 (MQ=255) cAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCg > 1:309580/1‑90 (MQ=255) aCGAATCGGTCCCAAAACCATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCaa < 2:163056/90‑1 (MQ=255) ccaaaaCAATAACCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGAc < 2:541063/87‑1 (MQ=255) ccATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGt > 2:294684/1‑90 (MQ=255) | GCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACCATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGT > NZ_CP009273/3703245‑3703414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |