| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,977,450 | 0 | T | C | 55.6% | 9.1 / 19.6 | 18 | F378S (TTC→TCC) | aslB | anaerobic sulfatase maturase |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base C (0/10); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACACCGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATT > NZ_CP009273/3977378‑3977520 | aaCCCCGCTTCATGCTCGATGCCAGCGGCAACCCGGGACTAAATTTTTTGTGTGCGGGGTATCAGCTTTTTTCCCCCCATCTACCGCCat < 1:535418/90‑1 (MQ=255)aaCACCGCTTCTTGCTCGATGCCAGCGGCAACCCGGGACTGAATTTTTTGTGTCCGGGGTATCAGCGTTTTTCCCCCCATCTACCGCCat < 2:360819/90‑1 (MQ=255) ccGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCt > 1:238764/1‑90 (MQ=255) ccGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCt > 1:101804/1‑90 (MQ=255) tGCTCGATCCCAGCGGCAACCCGGGATTAAATTTTTTGTGTGCCGGGTATCAGCTTTTTTCCCCCCATCTACCGCCATATCTTAAAGctg < 1:5711/90‑3 (MQ=255) aGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTg > 2:125494/1‑90 (MQ=255) gCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGc > 2:508872/1‑90 (MQ=255) ccGGGACTGAATTTTTTGTGTGCCGGGTATCAGTGTTATTCCCCCCCTCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCAc < 2:271819/90‑1 (MQ=255) gACTGAATTATTTGTGTGCGGGGTATCAGCGTTATTCCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTc < 2:37211/90‑1 (MQ=255) tttttttGTGTGCGGGGTGTCGGCTTTTTTCCCCCCATCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCgg < 1:519861/86‑1 (MQ=255) attattTGTGGGCGGGGTATCAGCTTTTTTCCCCCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCgg < 1:85974/90‑1 (MQ=255) ttGTGTGCGGGTTATCAGCGTTATTTCCCCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGc < 2:386590/90‑1 (MQ=255) ttGTGGGCCGGGTATCAGTTTTTTTCCCCCCTCCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCcggccggc < 1:438620/90‑1 (MQ=255) tgtgtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCg > 1:430280/1‑90 (MQ=255) tgtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCg < 2:337050/88‑1 (MQ=255) tgtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCg > 1:337050/1‑88 (MQ=255) tgCGGGGTATCGGTTTTTTTCCCCCCATCTCCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACAt < 1:125494/90‑1 (MQ=255) gcggggTATCAGCTTTTTTCCCCCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACAtt < 2:499985/87‑1 (MQ=255) | AACACCGCTTCATGCTCGATGCCAGCGGCAAACCGGGACTGAATTATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATT > NZ_CP009273/3977378‑3977520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |