| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 297,075 | 0 | A | C | 53.8% | 9.7 / 13.0 | 13 | V175G (GTG→GGG) | paoB | aldehyde oxidoreductase FAD‑binding subunit PaoB |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCAATGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCC > NZ_CP009273/296987‑297145 | ttCAATGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCcaccac < 2:512239/90‑1 (MQ=255) gcggcgTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCa > 1:48014/1‑90 (MQ=255) cGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGc > 1:82370/1‑90 (MQ=255) ggggggTAAAAAAAACCAACCGGTGTCTCACGGCGTTTCTTTCCCCCGGGGGTGTTGGTTCCCCCCCCCACACCCAGCAACCGCATTGCg < 1:364573/87‑1 (MQ=255) gTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGc > 2:49425/1‑90 (MQ=255) aaaaaTCAGCCGGTGTGATACTGCGAGTCTTCCCCTCCGGCGTGATGGTTTCCCCCCCCGCATCCAGCAACCGCATTGCGACCGCCatat < 2:516549/90‑1 (MQ=255) aaaaaTCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCatat < 2:418252/90‑1 (MQ=255) aaaaaTCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCatat < 2:346182/90‑1 (MQ=255) ccAGCCGGTGTGATACTGCGAGTCTTCCCCCCGGGCGTGGTGGTTCCCCCCCCCGCACCCAGCACCCGCTTTGCGACCGCCATATCGCTc < 1:443352/89‑1 (MQ=255) cAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCg > 1:143384/1‑90 (MQ=255) ttCTTCCCCCCCGGCGTGATGTTTCCCACCCCCGCATCCAGCACCCGCATTGCAACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCt < 2:274028/89‑1 (MQ=255) tCCCCCCGGCGTGATGTTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCt < 2:82370/90‑1 (MQ=255) cccccGGGGTGATGTTTCCCCCCCCCGCACCCAGCACCCGCTTGGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 2:370900/87‑1 (MQ=255) cGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGcc > 2:143228/1‑90 (MQ=255) | TTCAATGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCC > NZ_CP009273/296987‑297145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |