| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 58.3% | 5.9 / 10.1 | 12 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (7/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152646‑1152816 | gCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACt < 1:253365/90‑1 (MQ=255) cTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGTAATTTACt > 2:423321/1‑90 (MQ=255) aagaaAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTAATTGATCTCGGGTTTTTCa > 2:267614/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATACTGGAGCTCCGATTTTACATCTCCCTTTTCGGCGATG‑TGGACCTCCCGaaagggg > 2:129000/1‑86 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATTGATTTCGGATTTTAAATCTCCTTTTTCCGCATTGTT‑GACTTCCCCTAATggg > 1:14426/1‑88 (MQ=255) ttttACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGa < 2:224490/90‑1 (MQ=255) gagGACAGAGAAACGGC‑GGGGAAAATAATTGGTCTCGGGTTTTAAATCTCCTTTTTCCGCCTTG‑T‑GACCTTTCCTAATGCGGGGCAACTg > 1:180326/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATACTTGGTCTCGGGTTTTTCATTTCCTTTTTCCGCCTTT‑T‑TACCTTCCGTAAATCGGGGCAAATgcg > 2:483698/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATACTTGATCTCGGGTTTTAAATATCCTTTTTCCGCATTG‑T‑GACCTTCCGTAATGCGGAGCAAATgcg > 2:131364/1‑90 (MQ=255) gACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTgcgc < 1:483698/90‑1 (MQ=255) gACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTgcgc < 2:419573/90‑1 (MQ=255) gagaAACGGC‑GGGGAAAATATTGGGTTTCGGGTTTTTTATCTCCTTTTTCCGCGTTT‑T‑GGCCTTTCGTAATGGGGGGGAAATGCGCGagg > 2:304978/1‑88 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:131364/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:275863/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:91180/90‑1 (MQ=255) | GCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTG‑T‑GACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152646‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |