Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,218,0350GC55.6% 6.3 / 10.5 9A21G (GCG→GGG) ymgDYmgD family protein
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base C (0/5);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTG  >  NZ_CP009273/1217961‑1218122
                                                                          |                                                                                       
ttGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTg                                                                          >  1:367219/1‑90 (MQ=255)
                                     ttCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAAtttttt                                     >  1:436902/1‑90 (MQ=255)
                                     ttCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTCGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAAtttttt                                     >  2:356552/1‑90 (MQ=255)
                                      tCTGCCGGAAACCCTTTTTTAATTTTGTAGTCCTGCCCCCAGGTCTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTtc                                    <  2:367219/90‑1 (MQ=255)
                                      tCTGACGGAAACCCTTTTTTAATATTGTAGCCCTGCCCCCAGGTCATTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTtc                                    <  2:442997/90‑1 (MQ=255)
                                                       tttAATTTTGTAGCCCTGCCCCAAGGTTTTTGGTGAAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                   <  1:463466/90‑1 (MQ=255)
                                                            tatTGTAGCCCTCCCCCAAGTTTTTTGGTGAAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAttt              <  1:324862/90‑1 (MQ=255)
                                                                     ccTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGaa     >  1:203330/1‑90 (MQ=255)
                                                                      cTGCCCCCAGGTATTGGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt    <  2:436902/90‑1 (MQ=255)
                                                                        gcgcCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTg  >  2:136345/1‑90 (MQ=255)
                                                                          |                                                                                       
TTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTG  >  NZ_CP009273/1217961‑1218122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑