| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,661 | 0 | A | C | 73.7% | 2.1 / 10.8 | 19 | G142G (GGT→GGG) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/14); total (5/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.60e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAT > NZ_CP009273/3571577‑3571744 | cgggCGTGCTGCTTAAAATTTAAAAAAC‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGCCCATCAGTCCCCCCAGGGGAATCCCCGc < 1:131106/88‑1 (MQ=255) cGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACt > 1:477074/1‑90 (MQ=255) gctTAAAATTTAAAAAAC‑CCGGGGGCGATAACAGGGCAGGGCGCCTTGCTGTCCATCATTCCCCCCCGGGGAATCCCCGCCACTTCAatc < 2:477074/90‑1 (MQ=255) gctTAAAAGTTAAAAAAC‑CCGGGGGCGATACCAAGGCATGGAGATTTGCTTCCAATCAGTCCCCCCACGGAAATCCCCGCCACTTCAatc < 2:327570/90‑1 (MQ=255) gcttaaaaTTAACAAAACCCGGGGGCGATATAAGGGCAGGGCGACTTGCTGCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAatc < 2:303231/86‑1 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAgg > 1:64602/1‑90 (MQ=255) aacccgccgGCTATATCAAGGCAGGGCGCTTTGCTGCCCATCAGTCCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 1:312751/87‑1 (MQ=255) cccGAGGGCGATATAAAGGCAGGGCGTCTTGCTGTCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 2:64602/89‑1 (MQ=255) gccGGCAATATAAAGGCATGGCGTTTTGCTGTCCTTCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgt < 2:316371/88‑1 (MQ=255) gACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgt < 2:74666/90‑1 (MQ=255) aTGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa > 2:370485/1‑90 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 2:22668/1‑90 (MQ=255) cTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAg > 2:103496/1‑90 (MQ=255) cATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 2:182295/90‑1 (MQ=255) cATCAGCCCCCCCGCGGAAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:492689/90‑1 (MQ=255) cATCAGCCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 2:449222/90‑1 (MQ=255) gggAATCCCCGCCACTTCAATCATTTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTTCAGGAGAGCAt < 1:93822/90‑1 (MQ=255) gggAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt < 1:460756/90‑1 (MQ=255) ggaaaTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt < 1:289230/87‑1 (MQ=255) | CTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAT > NZ_CP009273/3571577‑3571744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |